FastQCFastQC Report
Sun 11 Jun 2023
EGAF00008066525

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008066525
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences414041
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGTGCCAGGCTGGGTATTCTAGAAACTTATCTCGTATGCCGTCTTCTGC67771.636794423740644RNA PCR Primer, Index 10 (96% over 26bp)
ACGTGCCAGCACGATCCAATCTCGCATATTATCTCGTATGCCGTCTTCTG21830.5272424711562381RNA PCR Primer, Index 44 (96% over 25bp)
ACGTGCCACCTGGGGAGCGTTATCTCGTATGCCGTCTTCTGCTTGAAAAA15320.37001166551138653Illumina PCR Primer Index 5 (96% over 27bp)
ACGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTAGGACAC6280.15167580022268326No Hit
ACGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTCCTGAAT5410.13066338840839434No Hit
TTGGCCATCTCCTGATAGAATCTCTTATCTCGTATGCCGTCTTCTGCTTG4200.10143922944829135TruSeq Adapter, Index 10 (96% over 28bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)