FastQCFastQC Report
Sun 11 Jun 2023
EGAF00008066526

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008066526
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences405487
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TACGTGCCAGGCTGGGTATTCTAGAAACTTATCTCGTATGCCGTCTTCTG8360.2061718378147758TruSeq Adapter, Index 10 (96% over 25bp)
TACGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTAGGACA7360.18151013472688396No Hit
TACGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTCCTGAA6280.1548754953919608No Hit
TACGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTACAAGC6080.14994315477438241No Hit
TACGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTTCTAGT5830.14377772900240945No Hit
TACGTGCCAGCACGATCCAATCTCGCATATTATCTCGTATGCCGTCTTCT5640.13909200541570998RNA PCR Primer, Index 44 (95% over 24bp)
TACGTGCCAGCACGCGGCTGAGCTCCCGCAGGAAGTTATCTCGTATGCCG4540.11196413201902897No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)