FastQCFastQC Report
Sun 11 Jun 2023
EGAF00008066536

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008066536
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences317272
Sequences flagged as poor quality0
Sequence length100
%GC49

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTGCCAGCACGATCCAATCTCGCATATTATCTCGTATGCCGTCTTCTGC4850.15286567992132935RNA PCR Primer, Index 35 (96% over 27bp)
CGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTAGGACACT4340.1367911445069215No Hit
CGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTTCTAGTTG3380.10653319549156559No Hit
CGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTACAAGCTC3300.10401169974028593No Hit
CGTGCCAGCACGCGGCTGAGCTCCCGCAGGAAGTTATCTCGTATGCCGTC3250.10243576489573614No Hit
CGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTCCTGAATC3200.10085983005118636No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)