FastQCFastQC Report
Sun 11 Jun 2023
EGAF00008066538

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008066538
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences343702
Sequences flagged as poor quality0
Sequence length100
%GC49

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGTGCCAGGCTGGGTATTCTAGAAACTTATCTCGTATGCCGTCTTCTGC7200.2094837970102007RNA PCR Primer, Index 10 (96% over 26bp)
ACGTGCCAGCACGATCCAATCTCGCATATTATCTCGTATGCCGTCTTCTG5370.15623999860344134RNA PCR Primer, Index 44 (96% over 25bp)
ACGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTAGGACAC4490.1306364234133057No Hit
ACGTGCCAGCACGCGGCTGAGCTCCCGCAGGAAGTTATCTCGTATGCCGT4070.11841653525437733No Hit
ACGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTCCTGAAT3880.11288849061105259No Hit
ACGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTACAAGCT3450.1003776527340545No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)