FastQCFastQC Report
Sun 11 Jun 2023
EGAF00008066546

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008066546
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences310553
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACGTGCCACCTGGGGAGCGTTATCTCGTATGCCGTCTTCTGCTTGAAA6500.20930404794028715Illumina PCR Primer Index 5 (96% over 27bp)
GTACGTGCCAGCACGCGGCTGAGCTCCCGCAGGAAGTTATCTCGTATGCC5010.16132512002782132No Hit
GTACGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTAGGAC4220.13588662804738644No Hit
GTACGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTACAAG3480.11205816720495373No Hit
GTACGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTCCTGA3370.10851609870134889No Hit
GTACGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTTCTAG3350.10787208624614801No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)