FastQCFastQC Report
Sun 11 Jun 2023
EGAF00008066549

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008066549
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences298523
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGTGCCAGGCTGGGTATTCTAGAAACTTATCTCGTATGCCGTCTTCTGC7740.25927650465793256RNA PCR Primer, Index 10 (96% over 26bp)
ACGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTAGGACAC4100.1373428513045896No Hit
ACGTGCCAGCACGCGGCTGAGCTCCCGCAGGAAGTTATCTCGTATGCCGT3990.1336580430988567No Hit
ACGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTACAAGCT3530.11824884514761007No Hit
ACGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTCCTGAAT3500.11724389745513746No Hit
ACGTGCCAGCACGATCCAATCTCGCATATTATCTCGTATGCCGTCTTCTG3000.10049476924726068RNA PCR Primer, Index 44 (96% over 25bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)