FastQCFastQC Report
Sun 11 Jun 2023
EGAF00008066559

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008066559
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences575953
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACGTGCCAGCACGCGGCTGAGCTCCCGCAGGAAGTTATCTCGTATGCC38090.6613386856219171No Hit
GTACGTGCCAGCACGATCCTGAAATTCTGTTAACGAATCCTTCTCTTTAT9590.16650664203502719No Hit
GTACGTGCCACCTGGGGAGCGTTATCTCGTATGCCGTCTTCTGCTTGAAA8960.1555682494925801Illumina PCR Primer Index 5 (96% over 27bp)
GTACGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTAGGAC7920.13751122053361994No Hit
GTACGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTTCTAG7020.1218849454729813No Hit
GTACGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTCCTGA6980.12119044435917514No Hit
GTACGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTACAAG6510.11303005627195276No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)