FastQCFastQC Report
Sun 11 Jun 2023
EGAF00008066564

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008066564
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences395565
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TACGTGCCAGCACGATCCAATCTCGCATATTATCTCGTATGCCGTCTTCT20260.5121787822481767RNA PCR Primer, Index 44 (95% over 24bp)
TACGTGCCAGGCTGGGTATTCTAGAAACTTATCTCGTATGCCGTCTTCTG17720.4479668322526007TruSeq Adapter, Index 10 (96% over 25bp)
TACGACCCCCTCGCTCCAATTTCCCCTGTTATCTCGTATGCCGTCTTCTG8840.2234778102208234RNA PCR Primer, Index 25 (96% over 25bp)
TACGTGCCAGCACGATCCAATTTCCCCTGTTATCTCGTATGCCGTCTTCT6130.15496821002869313TruSeq Adapter, Index 25 (95% over 24bp)
TACGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTAGGACA5630.14232806239176873No Hit
TACGTGCCAGCACGATCCTATCTTGCACAGGTCTTCTGCCATTTCTTATC5620.14207525943903027No Hit
TACGTGCCAGCACGATCCAATCTTGCATAGGTCTTCTGCCATTTCTTATC4950.12513746160555156No Hit
TACGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTCCTGAA4700.11881738778708935No Hit
TACGTGCCACCTGGGGAGCGTTATCTCGTATGCCGTCTTCTGCTTGAAAA4510.11401413168505808RNA PCR Primer, Index 31 (96% over 28bp)
TACGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTTCTAGT4460.11275011692136565No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)