FastQCFastQC Report
Sun 11 Jun 2023
EGAF00008066566

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008066566
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences405193
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TACGTGCCAGCACGCGGCTGAGCTCCCGCAGGAAGTTATCTCGTATGCCG23140.5710858775941342No Hit
TACGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTAGGACA6380.15745582969103614No Hit
TACGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTCCTGAA6370.15720903371973355No Hit
TACGTGCCAGCACGATCCAATCTCGCATATTATCTCGTATGCCGTCTTCT6140.15153272637977458RNA PCR Primer, Index 44 (95% over 24bp)
TACGTGCCAGCACGATCCTGAAATTCTGTTAACGAATCCTTCTCTTTATC5320.1312954567329643No Hit
TACGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTTCTAGT5290.1305550688190566No Hit
TACGTGCCAGGCTGGGTATTCTAGAAACTTATCTCGTATGCCGTCTTCTG4420.1090838193157335TruSeq Adapter, Index 10 (96% over 25bp)
TACGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTACAAGC4330.10686265557401041No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)