FastQCFastQC Report
Sun 11 Jun 2023
EGAF00008066578

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008066578
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences538171
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGTGCCAGGCTGGGTATTCTAGAAACTTATCTCGTATGCCGTCTTCTGC22690.4216132047248923RNA PCR Primer, Index 10 (96% over 26bp)
ACGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTAGGACAC9780.18172662592373057No Hit
ACGTGCCAGCACGATCCAATCTCGCATATTATCTCGTATGCCGTCTTCTG9340.17355078590262202RNA PCR Primer, Index 44 (96% over 25bp)
ACGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTCCTGAAT7450.1384318367210422No Hit
ACGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTTCTAGTT6580.1222659712247594No Hit
ACGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTACAAGCT6060.11260361483617659No Hit
ACGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTGGAATTC5620.10442777481506807No Hit

[OK]Adapter Content

Adapter graph