FastQCFastQC Report
Sun 11 Jun 2023
EGAF00008066580

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008066580
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences519737
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACGTGCCAGCACGCGGCTGAGCTCCCGCAGGAAGTTATCTCGTATGCC18910.36383786415052227No Hit
GTACGTGCCACCTGGGGAGCGTTATCTCGTATGCCGTCTTCTGCTTGAAA10720.20625816518739284Illumina PCR Primer Index 5 (96% over 27bp)
GTACGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTAGGAC8360.16085058404539218No Hit
GTACGTGCCAGCACGATCCAATCTCGCATATTATCTCGTATGCCGTCTTC7240.13930122350342578RNA PCR Primer, Index 35 (95% over 24bp)
GTACGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTCCTGA6980.13429869337761213No Hit
GTACGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTTCTAG6900.13275945333890024No Hit
GTACGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTACAAG6730.1294885682566375No Hit
GTACGTGCCAGCACGATCCTGAAATTCTGTTAACGAATCCTTCTCTTTAT5770.11101768779209484No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)