FastQCFastQC Report
Sun 11 Jun 2023
EGAF00008066582

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008066582
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences431250
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACGTGCCACCTGGGGAGCGTTATCTCGTATGCCGTCTTCTGCTTGAAA17150.3976811594202899Illumina PCR Primer Index 5 (96% over 27bp)
GTACGTGCCAGCACGATCCAATCTCGCATATTATCTCGTATGCCGTCTTC10020.2323478260869565RNA PCR Primer, Index 35 (95% over 24bp)
GTACGTGCCAGGCTGGGTATTCTAGAAACTTATCTCGTATGCCGTCTTCT8920.2068405797101449TruSeq Adapter, Index 10 (95% over 24bp)
GTACGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTAGGAC6640.15397101449275363No Hit
GTACGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTCCTGA6100.14144927536231885No Hit
GTACGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTACAAG5980.13866666666666666No Hit
GTACGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTTCTAG5290.12266666666666667No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)