FastQCFastQC Report
Sun 11 Jun 2023
EGAF00008066584

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008066584
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences371091
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACGTGCCACCTGGGGAGCGTTATCTCGTATGCCGTCTTCTGCTTGAAA15860.42738843033110474Illumina PCR Primer Index 5 (96% over 27bp)
GTACGTGCCAGCACGATCCAATCTCGCATATTATCTCGTATGCCGTCTTC15680.422537868070096RNA PCR Primer, Index 35 (95% over 24bp)
GTACGTGCCAGGCTGGGTATTCTAGAAACTTATCTCGTATGCCGTCTTCT5440.14659477055493128TruSeq Adapter, Index 10 (95% over 24bp)
GTACGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTAGGAC4750.12800094855439773No Hit
GTACGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTTCTAG4490.12099458084405172No Hit
GTACGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTCCTGA4360.11749139698887874No Hit
GTACGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTACAAG4350.11722192130771159No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)