FastQCFastQC Report
Sun 11 Jun 2023
EGAF00008066586

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008066586
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences367762
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGTGCCAGCACGCGGCTGAGCTCCCGCAGGAAGTTATCTCGTATGCCGT21260.5780912655467395No Hit
ACGTGCCAGCACGATCCAATCTCGCATATTATCTCGTATGCCGTCTTCTG10980.29856265737079957RNA PCR Primer, Index 44 (96% over 25bp)
ACGTGCCAGGCTGGGTATTCTAGAAACTTATCTCGTATGCCGTCTTCTGC10740.2920366976468477RNA PCR Primer, Index 10 (96% over 26bp)
ACGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTAGGACAC6330.17212218771923146No Hit
ACGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTCCTGAAT5550.15091281861638778No Hit
ACGTGCCAGCACGATCCTGAAATTCTGTTAACGAATCCTTCTCTTTATCT5100.13867664413397796No Hit
ACGTGCCACCTGGGGAGCGTTATCTCGTATGCCGTCTTCTGCTTGAAAAA4600.12508089470907816Illumina PCR Primer Index 5 (96% over 27bp)
ACGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTTCTAGTT4150.11284472022666833No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)