FastQCFastQC Report
Sun 11 Jun 2023
EGAF00008066589

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008066589
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences453336
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TACGTGCCAGGCTGGGTATTCTAGAAACTTATCTCGTATGCCGTCTTCTG20760.4579384827148075TruSeq Adapter, Index 10 (96% over 25bp)
TACGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTAGGACA7600.16764607267016077No Hit
TACGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTCCTGAA6520.14382268339598003No Hit
TACGTGCCAGCACGATCCAATCTCGCATATTATCTCGTATGCCGTCTTCT6480.14294033564508443RNA PCR Primer, Index 44 (95% over 24bp)
TACGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTACAAGC5910.13036688019482237No Hit
TACGTGCCAGCACGATCCAATCTCGCATAACTTGTATCATGAGTTCTAGT5820.12838159775530733No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)