FastQCFastQC Report
Sun 11 Jun 2023
EGAF00008066599

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008066599
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences182
Sequences flagged as poor quality0
Sequence length105
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAATATCCTTAAGAGGGCGTTCAGCAGCCAGCTTGCGGCAAAACTGCG2915.934065934065933No Hit
ACCATCATGGCGACCATCCAAAGGATAAACATCATAGGCAGTCGGGAGGG2513.736263736263737No Hit
CAGTCCTTGACGGTATAATAACCACCATCATGGCGACCATCCAAAGGATA2312.637362637362637No Hit
CCAAAAAGAAAGGTATTAAGGATGAGTGTTCAAGATTGCTGGAGGCCTCC147.6923076923076925No Hit
CAAAGCATTGGGATTATCATAAAACGCCTCTAATCGGTCGTCAGCCAACG126.593406593406594No Hit
ATGCCGCTTTTCTTGGCACGATTAACCCTGATACCAATAAAATCCCTAAG94.945054945054945No Hit
TAAAGCTACATCGTCAACGTTATATTTTGATAGTTTGACGGTTAATGCTG73.8461538461538463No Hit
CAGTCCTTGACGGTATAATAACCATCATCATGGCGACCATCCAAAGGATA31.6483516483516485No Hit
AACAGCCATATAACTGGTAGCTTTAAGCGGCTCACCTTTAGCATCAACAG21.098901098901099No Hit
CAGTCCTTGACGGTATAATAACCACTATCATGGCGACCATCCAAAGGATA21.098901098901099No Hit
TAAAGCTACATCGTCAACGTTATACTTTGATAGTTTGACGGTTAATGCTG10.5494505494505495No Hit
CAGTCCTTGACGGTATAATAACCACCATCATGGCGACCATCCAAAGGTTA10.5494505494505495No Hit
TGCCAGCACGATCCAATCTCGCATACATCATTCGAAGGTCTGATGAGAAA10.5494505494505495No Hit
TGCCAGCACGATCCAATCTCGCATAACACCAACATGTGTCGGGCACCCAT10.5494505494505495No Hit
TGCCAGCACGATCCAATCTCGCATAAAAGCAGGAGAGAAGACTGCCTAGA10.5494505494505495No Hit
TGCCAGCACGATCCAATCTCGCATAAGAAATCTAGGAAGGGAGGGTACCA10.5494505494505495No Hit
TGTGCCAGCACGATCCAATCTCGCATAACTTCTTTCTCCACGACAATCCT10.5494505494505495No Hit
TGCCAGCACGATCCAATCTCGCATACGAGCTCCGCCTCCCTGCTCCCAGC10.5494505494505495No Hit
TGCCAGCACGATCCAATCTCGCATAATAGGACGCCCATCTGAATCATCTT10.5494505494505495No Hit
TGCCAGCACGATCCAATCTCGCATAGTAGCTAATCAAAACTACAAGGATT10.5494505494505495No Hit
TGCCAGCACGATCCAATTTCGCATAATTACTCCAGAGCCCCACCTGAGGG10.5494505494505495No Hit
TGCCAGCACGATCCAATCTCGCATACCATTATGCCAGCAGCAGGACCACG10.5494505494505495No Hit
TGCCAGCACGATCCAATCTCGCATAGTTCAACCTCCAGCACTGCAGAGAC10.5494505494505495No Hit
TGTGCCAGCACGATCCAATCTCGCATATTTTTTCTCCCCTCGTTCACCCT10.5494505494505495No Hit
TGCCAGCACGATCCAATCTCGCATAAAACGAGCTGGCACCAGAGGTGAGC10.5494505494505495No Hit
TGCCAGCACGATCCAATCTCGCATAAGTGCAATCACAGAGCAGAAGGCTT10.5494505494505495No Hit
TGTGCCAGCACGATCCAATCTCGCATATGTGGCTTCGCCCTGAACATGGT10.5494505494505495No Hit
TGCCAGCACGATCCAATCTCGCATAGGTAGTAGGACACAGAGGAACTCTG10.5494505494505495No Hit
TGTGCCAGCACGATCCAATCTCGCATACATTCAACCGGCGAGAACATGGT10.5494505494505495No Hit
TGCCAGCACGATCCAATCTCGCATACTAAAACAAAGGCAAATGTTCTTTC10.5494505494505495No Hit
TGCCAGCACGATCCAATCTCGCATAGATACTGTACATGGGTAGTTTTCAC10.5494505494505495No Hit
TGCCAGCACGATCCAATCTCGCATATATATTCACGTCACGCTTGAAGACC10.5494505494505495No Hit
TGCCAGCACGATCCAATCTCGCATAACACCTCACGGCGAGAACATGGTGC10.5494505494505495No Hit
TGCCAGCACGATCCAATCTCGCATACTTCGTGAGATGTCTGTGCTCGCCT10.5494505494505495No Hit
TGCCAGCACGATCCAATCTCGCATATAACGTACGGTTTACCAGCACCAAG10.5494505494505495No Hit
TGCCAGCACGATCCAATCTCGCATACGTCTATATCTTCCACCTCATGGTT10.5494505494505495No Hit
TGCCAGCACGATCCAATCTCGCATAAAAATAGGATTCCACATACCTGGTC10.5494505494505495No Hit
TGACCACCCTTACCCTTGTAAAATCAAGTTATCTCGTATGCCGTCTTCTG10.5494505494505495Illumina PCR Primer Index 5 (95% over 24bp)
GCCAGCACGATCCAATCTCGCATACCTAGTGAACCTGGCTGTCCGAGAGT10.5494505494505495No Hit
TGTGCCAGCACGATCCAATCTCGCATAACACGTACCCCCACGAACATCCT10.5494505494505495No Hit
TGCCAGCACGATCCAATCTCGCATACGACGATCCGGCGGAACATGGTGCG10.5494505494505495No Hit
TTTGCCATCCCTCTCCCCTCTCTCCTCCTTCTTTCCTTCTCTCCCCTTCT10.5494505494505495No Hit
TGCCAGCACGATCCAATCTCGCATACCACAACGCGGCGAGAACATGGTGC10.5494505494505495No Hit
TGCCAGCACGATCCAATCTCGCATATAAACAGAGTGGATATCCTGGCCAC10.5494505494505495No Hit
TGCCAGCACGATCCAATCTCGCATATTTCTCCCATCATGCCGAACAGGCT10.5494505494505495No Hit
ACCATCATGGCGACCATCCCAAGGATAAACATCATAGGCAGTCGGGAGGG10.5494505494505495No Hit
ACCATCATGGCGACCATACAAAGGATAAACATCATAGGCAGTCGGGAGGG10.5494505494505495No Hit
TGCCAGCACGATCCAATCTCGCATAGAAAGTCACGGCGAGAACATGGTGC10.5494505494505495No Hit
TGTGCCAGCACGATCCAATCTCGCTTATCTTCACATTCTGTTACTTCCAC10.5494505494505495No Hit
TGCCAGCACGATCCAATCTCGCATAACTACTCCCGGCGAGAACATGGTGC10.5494505494505495No Hit
TGCCAGCACGATCCAATCTCGCATAGGTGGATTCTAGAGTCTTGGCCACT10.5494505494505495No Hit
ACAGCAATTCAATGAGATATTACTATCCTTATTTCATAGAAAATGAAACT10.5494505494505495No Hit
ATGCCGCTTTTCTTGGCACGATTAATCCTGATACCAATAAAATCCCTACG10.5494505494505495No Hit
CAAAGCATTGGGATTATCATAAAATGCCTCTAATCGGTCGTCAGCCAACG10.5494505494505495No Hit
TGCCAGCACGATCCAATCTCGCATACCTCAAAGGGAACGAGAGTATATTT10.5494505494505495No Hit
TGCCAGCACGATCCAATCTCGCATAAAAAGATTAACCACGAAAACCCTCA10.5494505494505495No Hit
TGCCAGAAAGCCGTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA10.5494505494505495RNA PCR Primer, Index 31 (96% over 28bp)
TGCCAGCACGATCCAATCTCGCATATAATATGAGTTTAGGAGCCAGGAAG10.5494505494505495No Hit
TGCCAGCACGATCCAATCTCGCATACAATCAAGATCACTCCTCATGCCAT10.5494505494505495No Hit
TGCCAGCGCGATCCAATCTCGCATAGGACTAGCAACCACGAAAACCCTCA10.5494505494505495No Hit
TGCCAGCACGATCCAATCTCGCATATTAAGTTCGAAAAAGGAGGAGAGAG10.5494505494505495No Hit
TAAAGCTACATCGTCAACGTTATAGTTTGATAGTTTGACGGTTAATGCTG10.5494505494505495No Hit
TGCCAGCACGATCCAATCTCGCATAGTACCACAGCCATCTGAATCATCTT10.5494505494505495No Hit
TGCCAGCACGATCCAATCTCGCATATTTCTTAACTGGAAGCTGGGACTGG10.5494505494505495No Hit
TGCCAGCACTATCCTCGCATAACAGTAGTTAGAATGACCTACATCAAAGA10.5494505494505495No Hit
TGCCAGCACGATCCAATCTCGCATAGCTGCATGCAACTCTCCCCTTCTCA10.5494505494505495No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)