FastQCFastQC Report
Wed 28 Jun 2023
EGAF00008066676

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008066676
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences951700
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACTCGATTTGGAGCGATCCCAAAGAGCAGCTTCCCTGAACTTTACTTTACTTGTCGTCGCTGCTGGATAGAGGC21179722.25459703688137No Hit
AACTCGATTTGGAGCAAGTGCAGTCCCAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCG484945.0955132920037824TruSeq Adapter, Index 2 (100% over 45bp)
AACTCGATTTGGAGCGATCGTGTAGAGCAAGTGCAGTCCCAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA125631.3200588420720816Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AACTCGATTTGGAGCGAGTGCAGTCCCAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCG56480.5934643269938006TruSeq Adapter, Index 2 (100% over 45bp)
AACTCGATTTGGAGCGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTTCTGC41540.4364820846905537TruSeq Adapter, Index 2 (100% over 60bp)
AACTCGATTTGGAGCGATCGGAAAAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTTCTGC23310.24493012503940315TruSeq Adapter, Index 2 (98% over 60bp)
AACTCGATTTGGAGCGATCACAATTGCTGAGCATCAAAAACAAAGAACCAAGACCCAGAGAGCCCTGGGACAGGG23070.2424083219501944No Hit
AACTCGATTTGGAGCGATCATGTCCCGCCGACACGCGAGCTCTGGCCCCGGCCCTGCCCCGGGAGCCTGCGGGTC19740.20741830408742248No Hit
AACTCGATTTGGAGCGATCTTGAGCTGCGGACGGTTCAGGGGCTCCAGCTCGTTCCCTTGGATCTGATGCAGTAG19370.2035305243248923No Hit
AACTCGATTTGGAGCGATCGCATCTTATTCAAAATTGTTCATCATCCCAGTGATAAGTGGTATCAGTGTAATATG17960.1887149311757907No Hit
AACTCGATTTGGAGCGATCATGACTTTCTTTCATCTCTTGCCTGGACTTCTATAGTGGTCTCCTAACTATTCCCT17810.18713880424503518No Hit
AACTCGATTTGGAGCGATCCTAAGGAAAAGAGGGTCTCAATATGACAAATCATATGAGTACCTTAGGCTTGAAGA16160.1698014080067248No Hit
AACTCGATTTGGAGCGATCCACCTCTTGACCACTTTCTCAACTATTCTCTCGGCCATAGTACTAACACGCAATAC15950.16759483030366712No Hit
ACTCGATTTGGAGCGATCCCAAAGAGCAGCTTCCCTGAACTTTACTTTACTTGTCGTCGCTGCTGGATAGAGGCT15860.16664915414521383No Hit
AACTCGATTTGGAGCGATCCAAGTGTCCATATGCAGCCCATAACTGTAGATGTAATTTGAGATGTAACTGAGACT14490.15225386151098036No Hit
AACTCGATTGGAGCGATCCCAAAGAGCAGCTTCCCTGAACTTTACTTTACTTGTCGTCGCTGCTGGATAGAGGCT14400.15130818535252705No Hit
AACTCGATTTGGAGCGATCTGATGCAGTAGGGCCATCCCGGATGCTTTGGTGTGGAGGGTCATGTCCCGCCGACA14380.15109803509509298No Hit
AACTCGATTTGGAGCAAGTGCAGTCCCAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATTTCG13360.14038037196595568TruSeq Adapter, Index 2 (97% over 45bp)
AACTCGATTTGGAGCGATCAACACCCCGTAGGTTCATTTCCCCCACCGCCCCCAATCGGTGTTAGCCTCAGTGAG13230.13901439529263424No Hit
AACTCGATTTGGAGCGATCCTTAGATGCCACTGGAAGAATGTGGGAAGTTTGTGAATCTCTCTTTCTCAGGAACA12600.13239466218346116No Hit
AACTCGATTTGGAGCGATCTGATGCAGTAGGGCCATCCCGGATGCTTTGGTGTGGAGGGTCATGGTGGAAGGCAA11860.12461910265840076No Hit
AACTCGATTTGGAGCGATCTGCAAAAGTTGGAATGATTCTGAAGGCTCCTGCCTAAGGGCATCTGTAGTGAAGGA11790.12388357675738153No Hit
AACTCGATTTGAGCGATCCCAAAGAGCAGCTTCCCTGAACTTTACTTTACTTGTCGTCGCTGCTGGATAGAGGCT11500.12083639802458758No Hit
AACTCGATTTGGAGCGATCCCAAAGAGCAGCTTCCCTGAGCTTTACTTTACTTGTCGTCGCTGCTGGATAGAGGC11440.12020594725228538No Hit
AACTCGATTTGGAGCGATCTCCAGCTTTGTTCATTCGGTTCTAATATGACACTCCCCAAAAATGTCCGCCTCTTT11200.11768414416307658No Hit
AACTCGATTTGGAGCGATCGCCATTCTAACTGGCGTGAGACGGTATTTCATTGTGGTTTTGATTTGCAAGGAAAT11020.11579279184617002No Hit
AACTCGATTTGGAGCGATCAGTGAAACCAATGTTGCTTTTCTATCAAGAGTCCAACCCTTTTCTAAGAAGGGTTG10940.11495219081643375No Hit
AACTCGATTTGGAGCGATCGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTTCTGCT10890.11442681517284858TruSeq Adapter, Index 2 (98% over 57bp)
AACTCGATTTGGAGCGATCTGCAGCTCTGCAGTTCAGCAACTAGACCCCATACCCTATGTTTCTCAAACATTCCC10710.11253546285594201No Hit
AACTCGATTTGGAGCGATCCCAAAGGGCAGCTTCCCTGAACTTTACTTTACTTGTCGTCGCTGCTGGATAGAGGC10430.10959335925186509No Hit
AACTCGATTTGGAGCGATCAAGTGGACTTCATCCCTGGGATGCAAGGCTGGTTCAATACACGCAAATCAATAAAT10380.1090679836082799No Hit
AACTCGATTTGGAGCGATCGGAAGAGCACACGTCTGAACTCCAGTGAGCAAGTGCAGTCCCAGAGATCGGAAGAG10360.10885783335084584Illumina Multiplexing Index Sequencing Primer (96% over 32bp)
AACTCGATTTGGAGCGATCTTATATTCCTGCGGTCAAAAAGAATACTAAAGGGCTTCTAGACCAGCACTCTAATT10300.10822738257854367No Hit
AACTCGATTTGGAGCGATCATGGCAGAGAGGGGCGAGGAGAGGCGCAGAAGGGCTGGAGCCTCGGCCCTGAGAAG10040.1054954292319008No Hit
AACTCGATTTGGAGCGATCTATTTCTCTCTCTTTGTGGTTCCAGTCTCATAGAAAACATAACTATGTGAAAACAC9900.10402437742986234No Hit
AACTCGATTTGGAGCGATCCTAGCAAGTGAAGCTCTGTCAATCTGGAGCCGCAGATTTTGACCACGTGTGCACAG9890.10391930230114531No Hit
AACTCGATTTGGAGCGATCAAAGGCTATAAAGTAGCAGATATAGGATGAACAAGTCTAGAGATTTAATCTACAAC9850.1034990017862772No Hit
AACTCGATTTGGAGCGATCTGCCCTTCCTTGGCCTCCCAAAGTGCTAGGATTATAGGTGTGGGTCACAGCACCCG9840.10339392665756017No Hit
AACTCGATTTGGAGCGATCCCAAAGAGCAGCCTCCCTGAACTTTACTTTACTTGTCGTCGCTGCTGGATAGAGGC9800.10297362614269204No Hit
AACTCGATTTGGAGCGATCCCAAAGAGCAGCTTCCCTGAACTTTACCTTACTTGTCGTCGCTGCTGGATAGAGGC9750.10244825049910687No Hit
AACTCGATTTGGAGCGATCATACAACTCTGGCTAATTTTACTTCCTGTCTGGGGTACTTTTTCTCAGTAAAAGTT9610.10097719869706841No Hit
AACTCGATTTGGAGCGATCACTTGAGCCCAGGAGTTCCAGACATGATCCAGCCATCCCATTACTGGGTATATACC9580.10066197331091731No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)