FastQCFastQC Report
Sun 11 Jun 2023
EGAF00008066677

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008066677
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1082016
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACTCGATTTGGAGCGATCCCAAAGAGCAGCTTCCCTGAACTTTACTTTACTTGTCGTCGCTGCTGGATAGAGGC16938915.654944104338567No Hit
AACTCGATTTGGAGCAAGTGCAGTCCCAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCG734846.79139680004732TruSeq Adapter, Index 3 (100% over 45bp)
AACTCGATTTGGAGCGAGTGCAGTCCCAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCG89070.8231856090852631TruSeq Adapter, Index 3 (100% over 45bp)
AACTCGATTTGGAGCGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCCGTCTTCTGC61280.5663502203294591TruSeq Adapter, Index 3 (100% over 60bp)
AACTCGATTTGGAGCGATCTGATGCAGTAGGGCCATCCCAGATGCTTTGGTGTGGAGGGTCATGTCCCGCCGACA50540.4670910596516133No Hit
AACTCGATTTGGAGCGATCTTGAGCTGCGGACGGTTCAGGGGCTCCAGCTCGTTCCCTTGGATCTGATGCAGTAG49870.4608989146186378No Hit
AACTCGATTTGGAGCGATCGTGTAGAGCAAGTGCAGTCCCAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA41130.38012376896459943Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AACTCGATTTGGAGCGATCGGAAAAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCCGTCTTCTGC31470.29084597639960963TruSeq Adapter, Index 3 (98% over 60bp)
AACTCGATTTGGAGCGATCCAGCAGGGTAGGCTTGTTTTGATTTCTCTCTCTGTAGCTTTAGCATTTTGAGAAAG31080.28724159346996714No Hit
AACTCGATTTGGAGCGATCCCTCATAGAAAGAACATCTTTAAACAACAGAGTCCGGGGCAGAGGGAATCAAAAAC28500.2633972140892556No Hit
AACTCGATTTGGAGCGATCAGTATGAGTATATTTTAGAGACGAAGTAGCGAACAGCTCAGACATACTTTTTTTTT25710.23761201313104427No Hit
AACTCGATTTGGAGCGATCTTCTCGGTTCACTTGGGCTAGGATATGCAGAATCAAATATCCAGATGAAAAACAAA24890.23003356697128322No Hit
AACTCGATTTGGAGCGATCCAGGTTGTATTTCAGCAGTTCTGACAATTGCTCTGGGTCGAAGCTTGAATCAGTAG24830.229479046520569No Hit
AACTCGATTTGGAGCGATCTTTCCTGCTTTCTCTTACAGGAATTTAGTGCTATAAATTTCTCTCTACACACTGCT21780.2012909236092627No Hit
AACTCGATTTGGAGCGATCAATCTTTACCCTTCTTCACCTGAGAGAGCCAGTAAGTCACGGCTGGAACGTGTGTG21630.19990462248247717No Hit
AACTCGATTTGGAGCGATCACAACCACTCCTTGTGTTTTTACTTTCATATATACATTGAAAATTTCAAAATAGAA20670.1910322952710496No Hit
AACTCGATTTGGAGCAAGTGCAGTCCCAGAGATCGGAAAAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCG20420.18872179339307366TruSeq Adapter, Index 3 (97% over 45bp)
AACTCGATTTGGAGCAAGTGCAGTCCCAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATTTCG19980.18465531008783606TruSeq Adapter, Index 3 (97% over 45bp)
AACTCGATTTGGAGCGATCGCATCTTATTCAAAATTGTTCATCATCCCAGTGATAAGTGGTATCAGTGTAATATG19760.18262206843521722No Hit
AACTCGATTTGGAGCGATCACAAGTCCTGATTAAGCCCAACCCTTCTATCGAGTAAATTGCTTTAAAATCTTTTA19030.1758754029515275No Hit
AACTCGATTTGGAGCGATCCTAAATTAGACTGTACACACATTTGCCATTTGAGTTCCAAGCATTTTAGCAATAGA17440.16118061100760062No Hit
AACTCGATTTGGAGCGATCGTGGGTATTGAGAAAGAGGAACATTTGCTGCTGCATCCGGAGATGTTTCCTTGAGA16800.15526572619998225No Hit
AACTCGATTTGGAGCGATCCAGCTAGAAGAGCTTTTCACCCGAGGCGGTCCAGGGTGGCAAAGCCATAGAGGAAA16320.1508295625942685No Hit
AACTCGATTTGGAGCGATCGGAAGAGCACACGTCTGAACTCCAGTGAGCAAGTGCAGTCCCAGAGATCGGAAGAG16280.15045988229379234Illumina Multiplexing Index Sequencing Primer (96% over 32bp)
AACTCGATTTGGAGCGATCCTAAAATGTGAGGTTCCAATGCTCTTAGGCTTTAACTAAGCAGAAAAACAAAACAA16160.14935084139236388No Hit
AACTCGATTTGGAGCGATCACCAGTGGAAAAATTAGTTGTCCAAAATATGCAGATGGCCAGGATACAAATTAGCA15840.1463933989885547No Hit
AACTCGATTTGGAGCGATCCAGCTCCTCCTCCTAGTGTTTTCCTCTGTGCTCTATGTGGCAAGCATTACTGGAAA15610.14426773726081685No Hit
AACTCGATTTGGAGCGATCGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCCGTCTTCTGCT15600.14417531718569782TruSeq Adapter, Index 3 (98% over 57bp)
AACTCGATTTGGAGCGATCTAACTCGATTTGGAGCAAGTGCAGTCCCAGAGATCGGAAGAGCACACGTCTGAACT15420.14251175583355513Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AACTCGATTTGGAGCGATCTATGTAGAAATTCCTGACTGTGGCTCTTTTAGAGGAGAGGGCAGTGGCTGAGCAGC15090.13946189335462691No Hit
AACTCGATTTGGAGCGATCAAATGCCTTACTGGCCCTAGCCCCCAGTCTCTGAACAGAGGCATTGCCATGTCCCG14450.13354700854700854No Hit
AACTCGATTTGGAGCGATCAATTTAGGAATACCCAACAGATGTTCGTGGCCAATAAGGTTGGCCTCATTATTTTA14380.1329000680211753No Hit
AACTCGATTTGGAGCGATCGGAGCAGCAGTTCCTATAAAATTAGAAACCATCTCAGATCAAATTAGATTCCTGAG14200.13123650666903264No Hit
AACTCGATTTGGAGCGATCTAGTTCGACTACTAAATATTTTCAAATGAAATCACCGATATTACATATTCAACATA14080.13012746576760417No Hit
AACTCGATTTGGAGCGATCCACCTCTTGACCACTTTCTCAACTATTCTCTCGGCCATAGTACTAACACGCAATAC13670.12633824268772367No Hit
AACTCGATTTGGAGCGATCCTCTAACTGCATTGTCTCTTTAAAACAAAATCTCTCTGAGGCCAGTTTAACGATGA13460.12439742111022387No Hit
AACTCGATTTGGAGCGATCTGCGTGGCTGTGGGATGCTCTAAATTTCTCCAAGGCGGCTGAAGATGCACACTGAA13430.12412016088486676No Hit
AACTCGATTTGGAGCGATCATCTGAGGTCAGGTGTTTGAGATAAGCCTGACCAACATGGTGGCACGTGCCTGTAG12930.1194991571289149No Hit
ACTCGATTTGGAGCGATCCCAAAGAGCAGCTTCCCTGAACTTTACTTTACTTGTCGTCGCTGCTGGATAGAGGCT12820.1184825363026055No Hit
AACTCGATTTGGAGCGATCAAATGAATCTCCATTTAAATTGAGGGGGCAGGAAGTCCTGTTCCTCTCCAATCCAC12790.11820527607724839No Hit
AACTCGATTTGGAGCGATCCCAAGTTACCAAAACCCTTATTTGATTATGAACTGCATAGTAGATACCAATACATC12430.11487815337296306No Hit
AACTCGATTTGGAGCGATCTTACTTTTTTATTAATTTAAATTGTTACTGATTACGTGACACTTTGTGCTGGTCTA12390.1145084730724869No Hit
AACTCGATTGGAGCGATCCCAAAGAGCAGCTTCCCTGAACTTTACTTTACTTGTCGTCGCTGCTGGATAGAGGCT12290.11358427232129654No Hit
AACTCGATTTGGAGCGATCACAAGGAGGTGGCATTGATGGCCGCAGATGTCCACCTTTCGTGTCCTTCTGCAGAG12030.11118135036820156No Hit
AACTCGATTTGGAGCGATCATTTGAAACTCTTTCTCAACTTTGAATAGAGACTGATTTCCAAAGTGAGATTTAAG12000.11090409014284447No Hit
AACTCGATTTGGAGCGATCTCTTCCCTCCCTTCAAACACAGGACAATGTTGTCGGTTCTGATTTTCCCATGATTG11950.1104419897672493No Hit
AACTCGATTTGGAGCGATCAGGGAACACTTCTACATTGCTGATGGGAATGTAAACTAGTACAACCGCTATGGAAA11600.10720728713808297No Hit
AACTCGATTTGGAGCGATCGGAAGAGCAAGTGCAGTCCCAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC11600.10720728713808297Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AACTCGATTTGGAGCGATCTTCACTTGGTGATTCCTTGTTTATTTATTTATTGGTGTACTGCCTGCCTCTCTTCA11470.1060058261615355No Hit
AACTCGATTTGGAGCGATCAGGAAAAATACAATAATGTTCCCTCTCACCACTCCTTTTCAACACCATACTAGAAG11360.1049892053352261No Hit
AACTCGATTTGGAGCGATCACGCTTTATCCTGTAAGTTTTTCTGACTACAACCTATAAATCATTAAACATAGTAC11300.10443468488451188No Hit
AACTCGATTTGGAGCAAGTGCAGTCCCAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCAGCTCG11220.10369532428355958TruSeq Adapter, Index 3 (97% over 45bp)
AACTCGATTTGGAGCGATCCCAGCTATGTTTCCCACAGTCTGTCCCCCTACCTAAGGGGTGATGAGAGAAATCCC11200.1035104841333215No Hit
AACTCGATTTGGAGCGATCCTCATGTGTAACACAGGGACAATGACTGTGCCTTGTCACAGCCTCGTTGTATGGAT11080.10240144323189306No Hit
AACTCGATTTGGAGCGATCAGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCCGTCTTCTGC10980.1014772424807027TruSeq Adapter, Index 3 (98% over 60bp)
AACTCGATTTGGAGCGATCCCAAACATTTCATAGTCCATGATGATTCCATTCCATTCCATTCAAGGATTCCATTC10980.1014772424807027No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)