FastQCFastQC Report
Wed 28 Jun 2023
EGAF00008066678

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008066678
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2112479
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACTCGATTTGGAGCGATCCCAAAGAGCAGCTTCCCTGAACTTTACTTTACTTGTCGTCGCTGCTGGATAGAGGC39282218.59530911313201No Hit
AACTCGATTTGGAGCAAGTGCAGTCCCAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCG26225312.414466605348503TruSeq Adapter, Index 4 (100% over 45bp)
AACTCGATTTGGAGCGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTCTGC539622.554439594429104TruSeq Adapter, Index 4 (100% over 60bp)
AACTCGATTTGGAGCGATCGGAAGAGCACACGTCTGAACTCCAGTGAGCAAGTGCAGTCCCAGAGATCGGAAGAG290611.3756823144750787Illumina Multiplexing Index Sequencing Primer (96% over 32bp)
AACTCGATTTGGAGCGAGTGCAGTCCCAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCG284161.345149466574579TruSeq Adapter, Index 4 (100% over 45bp)
AACTCGATTTGGAGCGATCGGAAGAGCAAGTGCAGTCCCAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC225401.0669928553135912Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AACTCGATTTGGAGCGATCTAACTCGATTTGGAGCAAGTGCAGTCCCAGAGATCGGAAGAGCACACGTCTGAACT155240.7348712105540458Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AACTCGATTTGGAGCGATCGTGTAGAGCAAGTGCAGTCCCAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA140650.6658054352256282Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AACTCGATTTGGAGCGATCGGAAGAGCACACGTCTGAACTCCAGGAGCAAGTGCAGTCCCAGAGATCGGAAGAGC133910.633899792613323Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
AACTCGATTTGGAGCGATCGGAAAAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTCTGC126260.5976864148708697TruSeq Adapter, Index 4 (98% over 60bp)
AACTCGATTTGGAGCGATCTACACGACGCTCTTCCGATCTAACTCGATTTGGAGCAAGTGCAGTCCCAGAGATCG125330.5932840042433557Illumina Single End PCR Primer 1 (95% over 23bp)
AACTCGATTTGGAGCGATCTTGAGCTGCGGACGGTTCAGGGGCTCCAGCTCGTTCCCTTGGATCTGATGCAGTAG125230.5928106267565263No Hit
AACTCGATTTGGAGCGATCTGATGCAGTAGGGCCATCCCGGATGCTTTGGTGTGGAGGGTCATGTCCCGCCGACA114180.5405024144618716No Hit
AACTCGATTTGGAGCGATCTGATGCAGTAGGGCCATCCCAGATGCTTTGGTGTGGAGGGTCATGTCCCGCCGACA98110.46443065232837816No Hit
AACTCGATTTGGAGCGATCTGGAGCAAGTGCAGTCCCAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTG82940.39261928757634984TruSeq Adapter, Index 2 (97% over 35bp)
AACTCGATTTGGAGCAAGTGCAGTCCCAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATTTCG71830.34002704878959744TruSeq Adapter, Index 4 (97% over 45bp)
AACTCGATTTGGAGCGATCATCTTAAGGCCCTTTTAAGATTTATGATTCTGGAATTCTGTGGCTCTATTTTTCCA50210.2376828361370693No Hit
AACTCGATTTGGAGCGATCGGAGCAAGTGCAGTCCCAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGA46230.218842412161257TruSeq Adapter, Index 2 (97% over 36bp)
AACTCGATTTGGAGCGATCGAGCAAGTGCAGTCCCAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGAC46180.21860572341784226TruSeq Adapter, Index 4 (100% over 37bp)
AACTCGATTTGGAGCGATCGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTCTGCT45370.21477136577452366TruSeq Adapter, Index 4 (98% over 57bp)
AACTCGATTTGGAGCAAGTGCAGTCCCAGAGATCGGAAAAGCACACGTCTGAACTCCAGTCACTGACCAATCTCG40460.19152853117119745TruSeq Adapter, Index 4 (97% over 45bp)
AACTCGATTTGGAGCAAGTGCAGTCCCAGAGATCGGAAGGGCACACGTCTGAACTCCAGTCACTGACCAATCTCG37660.17827396153997271TruSeq Adapter, Index 4 (97% over 45bp)
AACTCGATTTGGAGCAAGTGCAGTCCCAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAAGCTCG33410.15815541834972088TruSeq Adapter, Index 4 (97% over 45bp)
AACTCGATTTGGAGCGATCGGAAGAGGAGCAAGTGCAGTCCCAGAGATCGGAAGAGCACACGTCTGAACTCCAGT32420.15346898123010927Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AACTCGATTTGGAGCGATCAGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTCTGC32150.15219086201566975TruSeq Adapter, Index 4 (98% over 60bp)
AACTCGATTTGGAGCGATCCTGGAGCTCTGGGCTTCCATACCTGTGTGGGACAGGGAAGCTCTCTCGGTCTCCAT32120.1520488487696209No Hit
AACTCGATTTGGAGCGATCGGAGGAGCAAGTGCAGTCCCAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC30500.14438013348298376Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AACTCGATTTGGAGCGATCTCAGCTCACTGCAACCCCTGCCTCCCAGGTTCAAGTGATTCTCCTGCCTCAGCCTC30280.14333870301195892No Hit
AACTCGATTTGGAGCGATCTGGAGATAAAAACACTAGTCAGCTAAGCTCTGTGCTCTATATTTAAAACTGCACAG29320.13879427913839618No Hit
AACTCGATTTGGAGCGATCTCGGCTCACTGCAAGCTCCGACTCCCAGGTTCACGCCATTCTCCTGCCTCAGCCTC28440.13462855725429695No Hit
ACTCGATTTGGAGCGATCCCAAAGAGCAGCTTCCCTGAACTTTACTTTACTTGTCGTCGCTGCTGGATAGAGGCT27770.13145692809253962No Hit
AACTCGATTTGGAGCGATCCCTGTGGGAAGCGGTGGCCCAGTGTTAACTTTCATCTCCAGTAAAGGCAGAAATCC27060.1280959479360505No Hit
AACTCGATTGGAGCGATCCCAAAGAGCAGCTTCCCTGAACTTTACTTTACTTGTCGTCGCTGCTGGATAGAGGCT26840.1270545174650257No Hit
AACTCGATTTGGAGCGATCCTATTATAGTCATCTTTGCTTCTCAGTTTCCACCACGATACTAACAATAGCAGCCA26340.12468763003087842No Hit
AACTCGATTTGGAGCGATCCTCTTCTTAGACTTCTCATTTGTCATATTTATTCTTTAATCTTCCTTTCCCTCCCT26310.12454561678482959No Hit
AACTCGATTTGGAGCGATCCACGACGCTCTTCCGATCTAACTCGATTTGGAGCAAGTGCAGTCCCAGAGATCGGA26090.12350418631380476Illumina Single End PCR Primer 1 (95% over 21bp)
AACTCGATTTGGAGCGATCGGAAGAGCACACGTCTGAACTCCAGTAGCAAGTGCAGTCCCAGAGATCGGAAGAGC25900.12260476908882881Illumina Multiplexing PCR Primer 2.01 (96% over 31bp)
AACTCGATTTGGAGCGATCGGAAGAGAGCAAGTGCAGTCCCAGAGATCGGAAGAGCACACGTCTGAACTCCAGTC25400.12023788165468155Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AACTCGATTTGGAGCAAGTGCAGTCCCAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATATCG25360.12004853065994976TruSeq Adapter, Index 4 (97% over 45bp)
AACTCGATTTGGAGCGATCCTGCTTAGTCCAGAATGATGGACATAGGCTGGTATCTACTGAAGAGGAAAATGAAA25290.11971716641916913No Hit
AACTCGATTTGGAGCGATCTGATGCAGTAGGGCCATCCCGGATGCTTTGGTGTGGAGGGTCATGAGGTCAAGTGT25080.11872307369682729No Hit
AACTCGATTTGGAGCGATCTGATGCAGTAGGGCCATCCCGGATGCTTTGGTGTGGAGGGTCATGAATCAACTGTA24870.11772898097448542No Hit
AACTCGATTTGGAGCGATCCACCCCACTTGGCCTCCCAAAGTACTGGGATTACAGGCATGATGAAATTACTTCCC24630.11659287500609472No Hit
AACTCGATTTGGAGCGATCGCTTGAGATGAGTTTGAGACTGACTCAGGCAACATAGTGAGACTTCATCTCTACAA24390.11545676903770405No Hit
AACTCGATTTGGAGCGATCATGTTAAGTGAGTCTTTAAAAATGATTACATTTTGCTTCATCTCAATGGACTATGC23670.11204845113253196No Hit
AACTCGATTTGGAGCGATCACTCTTCAGTAAGAATTCTGATATTAAGTAGTTTAATTCAGATCCATGGTGAAACC23660.11200111338384902No Hit
AACTCGATTTGGAGCGATCGAAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTCTGC23640.11190643788648313TruSeq Adapter, Index 4 (98% over 60bp)
AACTCGATTTGGAGCGATCGGAAGAGCACACGTCTGAACTCCAGTGGAGCAAGTGCAGTCCCAGAGATCGGAAGA23060.1091608484628723Illumina Multiplexing PCR Primer 2.01 (96% over 31bp)
AACTCGATTTGGAGCGATCCACACTAAGGAAGTGATTACCACTCCATTCAGGTTTCTGTTTCCACTCACGTGCAT22900.10840344448394518No Hit
AACTCGATTTGGAGCGATCTCTGGGAGCAAGTGCAGTCCCAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA22860.10821409348921339Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AACTCGATTTGGAGCGATCACTGTAACAAATATAACAGTAAAGAAAAAGTTTGGAATATTGTGAGAATTACCAAA22550.10674662328004206No Hit
AACTCGATTTGGAGCGATCTAAAAATATACACATAATTTAAAACATTGTTGATTACTGCAAAACTTTCTTCAGAG22460.10632058354189555No Hit
AACTCGATTTGGAGCGATCAATCTGCTCTTGCTGTGACTGGGCCACTTCCACCTCTTGGTAGCCATTGTTTTGTA21650.10248622589857698No Hit
AACTCGATTTGGAGCGATCGGGAGCAAGTGCAGTCCCAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTG21360.10111343118677156TruSeq Adapter, Index 2 (97% over 35bp)
AACTCGATTTGGAGCGATCGGAAGAGCGGAGCAAGTGCAGTCCCAGAGATCGGAAGAGCACACGTCTGAACTCCA21200.10035602720784444Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)