FastQCFastQC Report
Sun 11 Jun 2023
EGAF00008066680

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008066680
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1775550
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACTCGATTTGGAGCGATCCCAAAGAGCAGCTTCCCTGAACTTTACTTTACTTGTCGTCGCTGCTGGATAGAGGC24775613.95376080651066No Hit
AACTCGATTTGGAGCAAGTGCAGTCCCAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCG19183110.804032553293345TruSeq Adapter, Index 6 (100% over 45bp)
AACTCGATTTGGAGCGAGTGCAGTCCCAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCG228061.2844470727380248TruSeq Adapter, Index 6 (100% over 45bp)
AACTCGATTTGGAGCGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGTCTTCTGC109250.6153023006955591TruSeq Adapter, Index 6 (100% over 60bp)
AACTCGATTTGGAGCGATCGTGTAGAGCAAGTGCAGTCCCAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA98020.5520542930359607Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AACTCGATTTGGAGCGATCCAGCAGGGTAGGCTTGTTTTGATTTCTCTCTCTGTAGCTTTAGCATTTTGAGAAAG65340.36799864830615864No Hit
AACTCGATTTGGAGCGATCTAACTCGATTTGGAGCAAGTGCAGTCCCAGAGATCGGAAGAGCACACGTCTGAACT62680.35301737489791896Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AACTCGATTTGGAGCGATCGGAAGAGCAAGTGCAGTCCCAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC55220.31100222466278055Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AACTCGATTTGGAGCGATCGGAAGAGCACACGTCTGAACTCCAGTGAGCAAGTGCAGTCCCAGAGATCGGAAGAG54670.30790459294303174Illumina Multiplexing Index Sequencing Primer (96% over 32bp)
AACTCGATTTGGAGCGATCTTGAGCTGCGGACGGTTCAGGGGCTCCAGCTCGTTCCCTTGGATCTGATGCAGTAG54340.30604601391118247No Hit
AACTCGATTTGGAGCAAGTGCAGTCCCAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATTTCG52960.29827377432344904TruSeq Adapter, Index 6 (97% over 45bp)
AACTCGATTTGGAGCGATCTTCTCGGTTCACTTGGGCTAGGATATGCAGAATCAAATATCCAGATGAAAAACAAA50370.2836867449522683No Hit
AACTCGATTTGGAGCGATCAGTATGAGTATATTTTAGAGACGAAGTAGCGAACAGCTCAGACATACTTTTTTTTT48770.2746754526766354No Hit
AACTCGATTTGGAGCGATCACCAGTGGAAAAATTAGTTGTCCAAAATATGCAGATGGCCAGGATACAAATTAGCA46400.26132747599335415No Hit
AACTCGATTTGGAGCGATCGGAAAAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGTCTTCTGC41120.23159021148376557TruSeq Adapter, Index 6 (98% over 60bp)
AACTCGATTTGGAGCGATCGCATCTTATTCAAAATTGTTCATCATCCCAGTGATAAGTGGTATCAGTGTAATATG40450.2278167328433443No Hit
AACTCGATTTGGAGCGATCTGATGCAGTAGGGCCATCCCGGATGCTTTGGTGTGGAGGGTCATGTCCCGCCGACA38810.21858015826082058No Hit
AACTCGATTTGGAGCGATCCTTCCTAGTCCATCTCCTCCGACTGTAACCTATGCTTCAGCCAAGCAAAATTTTAA35280.1986989946777055No Hit
AACTCGATTTGGAGCGATCGGAAGAGCACACGTCTGAACTCCAGGAGCAAGTGCAGTCCCAGAGATCGGAAGAGC32670.18399932415307932Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
AACTCGATTTGGAGCGATCTACACGACGCTCTTCCGATCTAACTCGATTTGGAGCAAGTGCAGTCCCAGAGATCG30900.17403058207316044Illumina Single End PCR Primer 1 (95% over 23bp)
AACTCGATTTGGAGCGATCACAATTGCTGAGCATCAAAAACAAAGAACCAAGACCCAGAGAGCCCTGGGACAGGG30290.17059502689307537No Hit
AACTCGATTTGGAGCGATCTGGAGCAAGTGCAGTCCCAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGC29660.16704683055954495TruSeq Adapter, Index 5 (97% over 35bp)
AACTCGATTTGGAGCGATCCAAATTAAATGAGTGTGAGAAAGATTAGAATAAGTGGCAATTATCCTTCAGGAGCT28850.16248486384500577No Hit
AACTCGATTTGGAGCGATCTAAAACTCCTGAAACCAAGTTTACTGGGAGACAACAGTCAAGACTACACTGTTAGT28770.16203429923122414No Hit
AACTCGATTTGGAGCGATCTTACTTTTTTATTAATTTAAATTGTTACTGATTACGTGACACTTTGTGCTGGTCTA26280.14801047562727043No Hit
AACTCGATTTGGAGCAAGTGCAGTCCCAGAGATCGGAAAAGCACACGTCTGAACTCCAGTCACGCCAATATCTCG24720.13922446565852836TruSeq Adapter, Index 6 (97% over 45bp)
AACTCGATTTGGAGCAAGTGCAGTCCCAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATAGCTCG24410.13747852778012445TruSeq Adapter, Index 6 (97% over 45bp)
AACTCGATTTGGAGCGATCCACCTCTTGACCACTTTCTCAACTATTCTCTCGGCCATAGTACTAACACGCAATAC23780.133930331446594No Hit
AACTCGATTTGGAGCGATCCTAAAATATTGTTAAAAGAAATTTTCCCAGAAAATGTAAGATAAAAGTTACTAAAT23530.13252231702852638No Hit
AACTCGATTTGGAGCGATCACAAGTCCTGATTAAGCCCAACCCTTCTATCGAGTAAATTGCTTTAAAATCTTTTA23220.1307763791501225No Hit
AACTCGATTTGGAGCAAGTGCAGTCCCAGAGATCGGAAGGGCACACGTCTGAACTCCAGTCACGCCAATATCTCG22250.12531328320802004TruSeq Adapter, Index 6 (97% over 45bp)
AACTCGATTTGGAGCGATCCTCGTGCGCCCCCGCCCCCTGGCCGTGAAACTCAGCCTCTATCCAGCAGCGACGAC21230.11956858438230407No Hit
AACTCGATTTGGAGCGATCACAGTAGCTTTCAATTCTACTCCTCAGCTTTCCAAAATAAGTTTCAAGACTGGCCG20650.11630199093238716No Hit
AACTCGATTTGGAGCGATCGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGTCTTCTGCT20440.11511925882121032TruSeq Adapter, Index 6 (98% over 57bp)
AACTCGATTTGGAGCGATCTAGCACTAGCTAGGAGACACTGTATTGTTGAACTGTGTTAGAATTTTATAAGCTCT19920.11219058883162962No Hit
AACTCGATTTGGAGCGATCATTACCACCACCACATCACTTACAAGCGGGTTGTAAGAAAGTCATACTCTTTCTTA19060.10734701923347693No Hit
AACTCGATTTGGAGCGATCACCCAGGTTGGAGTGCAGTGGTGCAATCTTGGCTCACTGCAACCTCCGCTTCCCAG18520.10430570809045084No Hit
ACTCGATTTGGAGCGATCCCAAAGAGCAGCTTCCCTGAACTTTACTTTACTTGTCGTCGCTGCTGGATAGAGGCT18190.10244712905860157No Hit
AACTCGATTTGGAGCGATCCTTAGATGCCACTGGAAGAATGTGGGAAGTTTGTGAATCTCTCTTTCTCAGGAACA17940.10103911464053393No Hit
AACTCGATTTGGAGCGATCAGAACATTTAAAAACTTCCCTGTGGATTTCTACCTCTTACCTAAGCTTTGTTCTTG17850.10053222945002956No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)