FastQCFastQC Report
Sun 11 Jun 2023
EGAF00008066682

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008066682
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1293894
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACTCGATTTGGAGCGATCCCAAAGAGCAGCTTCCCTGAACTTTACTTTACTTGTCGTCGCTGCTGGATAGAGGC20638115.950379242812781No Hit
AACTCGATTTGGAGCAAGTGCAGTCCCAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCG1246649.63479234002167TruSeq Adapter, Index 8 (100% over 45bp)
AACTCGATTTGGAGCGAGTGCAGTCCCAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCG149821.1579001061910792TruSeq Adapter, Index 8 (100% over 45bp)
AACTCGATTTGGAGCGATCTGATGCAGTAGGGCCATCCCAGATGCTTTGGTGTGGAGGGTCATGTCCCGCCGACA91090.7039989365434881No Hit
AACTCGATTTGGAGCGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCCGTCTTCTGC78760.6087051953251194TruSeq Adapter, Index 8 (100% over 60bp)
AACTCGATTTGGAGCGATCTTGAGCTGCGGACGGTTCAGGGGCTCCAGCTCGTTCCCTTGGATCTGATGCAGTAG70050.541389016410927No Hit
AACTCGATTTGGAGCGATCGGAAAAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCCGTCTTCTGC60520.46773537863225273TruSeq Adapter, Index 8 (98% over 60bp)
AACTCGATTTGGAGCGATCCAGCAGGGTAGGCTTGTTTTGATTTCTCTCTCTGTAGCTTTAGCATTTTGAGAAAG35320.2729744476750028No Hit
AACTCGATTTGGAGCAAGTGCAGTCCCAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATTTCG34640.26771899398250554TruSeq Adapter, Index 8 (97% over 45bp)
AACTCGATTTGGAGCGATCTTCTCGGTTCACTTGGGCTAGGATATGCAGAATCAAATATCCAGATGAAAAACAAA32750.25311192416071177No Hit
AACTCGATTTGGAGCGATCGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCCGTCTTCTGCT27790.21477802663896736TruSeq Adapter, Index 8 (98% over 57bp)
AACTCGATTTGGAGCGATCCTTAGATGCCACTGGAAGAATGTGGGAAGTTTGTGAATCTCTCTTTCTCAGGAACA24800.19166948760872218No Hit
AACTCGATTTGGAGCGATCGTGTAGAGCAAGTGCAGTCCCAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA24310.18788246950677567Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AACTCGATTTGGAGCGATCAGCCTGGATGACAGAGTGAGACCCTCATCTTTATGGATATATATGTATAAAGAACA21400.16539221914623609No Hit
AACTCGATTTGGAGCGATCATGTCCCGCCGACACGCGAGCTCTGGCCCCGGCCCTGCCCCGGGAGCCTGCGGGTC20880.16137334279314997No Hit
AACTCGATTTGGAGCGATCAGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCCGTCTTCTGC20250.15650431951921873TruSeq Adapter, Index 8 (98% over 60bp)
AACTCGATTTGGAGCGATCCTAGGAGGTCGAGGTTGCCCTGAGCTGTGGTTGTGCCACTGCACTTCAGCCTGGGG19360.14962585806874443No Hit
AACTCGATTTGGAGCGATCCTGCCTCCGCCCCATTCTACCATTCTCACTGCCAGAAAGCCAGCCTTTTTTCAAGG18970.14661170080392985No Hit
AACTCGATTTGGAGCGATCCACCTCTTGACCACTTTCTCAACTATTCTCTCGGCCATAGTACTAACACGCAATAC17940.13865123418147082No Hit
AACTCGATTTGGAGCGATCACAATTGCTGAGCATCAAAAACAAAGAACCAAGACCCAGAGAGCCCTGGGACAGGG17470.13501878824695068No Hit
AACTCGATTTGGAGCGATCCCTGAGGAATCGCCACACTGACTTCCACAATGGTTGAACTAGTTTACAGTCCCACC17340.13401406915867917No Hit
AACTCGATTTGGAGCGATCCCATCCAAAGCGGCTGCTGCAGCAGTTACAGCCGCCTCCTCCACACACAGGAGCAG17210.13300935007040762No Hit
AACTCGATTTGGAGCGATCAATAAGTGCTCAGGGAGGCTATGTCTAACTGTCATCCCAAACTTTGGTTTATGCCA16750.1294541902196007No Hit
AACTCGATTTGGAGCGATCATTTTTGAAAAAGAGTTTAGGACACATCCAATAGTAAGAGTGTAGTTTTATAAATC16690.12899047371732153No Hit
AACTCGATTTGGAGCGATCTGATGCAGTAGGGCCATCCCAGATGCTTTGGTGTGGAGGGTCATGGTCATGGTCCG16670.12883590154989513No Hit
AACTCGATTTGGAGCAAGTGCAGTCCCAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAAGCTCG16620.12844947113132915TruSeq Adapter, Index 8 (97% over 45bp)
AACTCGATTTGGAGCAAGTGCAGTCCCAGAGATCGGAAAAGCACACGTCTGAACTCCAGTCACACTTGAATCTCG16050.12404416435967706TruSeq Adapter, Index 8 (97% over 45bp)
AACTCGATTTGGAGCGATCATATGACAGAAGGAAGGGCTGAAGAGTGTGAGAAGCTAGACCTCTGCAGGTTACCG15980.1235031617736847No Hit
AACTCGATTTGGAGCGATCTGGCTCCTCCGTTGAATGTGTCTCCAGCCCCACTTTTCCAGCGGTCTGCTGGGCAT15940.12319401743883193No Hit
AACTCGATTTGGAGCGATCGTTATGGGCAGAGAGGAAGCCCTGAACTCATGATCTACACTCCAGCCTGGGTGACA15940.12319401743883193No Hit
AACTCGATTTGGAGCGATCGAGACCATCCTGGCTAACACGGTGAAACCCCATCTCTACTAAAAATACAAAAAATT15560.12025714625773054No Hit
AACTCGATTTGGAGCGATCACAATATCTTGATGATTGTGAAAGGGCACAAACAGGTGGAGGCAAAGATGGTCATT15190.11739756116034236No Hit
AACTCGATTTGGAGCGATCTAGAATCCCTAATTCTCCAGTACTGGTTGTATGACCTTGGGCAAGTCACTTCCCCT15160.11716570290920277No Hit
AACTCGATTTGGAGCGATCTCTCAATGAGGCTTACTCTAACTTGACCCTCACCCTGCCCTGGCACTGCTGTCTCC14960.11561998123493888No Hit
AACTCGATTTGGAGCGATCATTTGGATTTAGGTCCCACCCTAATCGAGTATGACTTCATCTTAACTTGATTGCAT14840.11469254823038054No Hit
AACTCGATTTGGAGCGATCTGCGTGGCTGTGGGATGCTCTAAATTTCTCCAAGGCGGCTGAAGATGCACACTGAA14800.11438340389552776No Hit
AACTCGATTTGGAGCGATCCACCCGCATCAGCCTCCCAAAGTGCTGGGATTACAGGTGTGAACCACTGTGCCCCG14350.11090553012843402No Hit
ACTCGATTTGGAGCGATCCCAAAGAGCAGCTTCCCTGAACTTTACTTTACTTGTCGTCGCTGCTGGATAGAGGCT14310.11059638579358125No Hit
AACTCGATTGGAGCGATCCCAAAGAGCAGCTTCCCTGAACTTTACTTTACTTGTCGTCGCTGCTGGATAGAGGCT14200.1097462388727361No Hit
AACTCGATTTGGAGCAAGTGCAGTCCCAGAGATCGGAAGGGCACACGTCTGAACTCCAGTCACACTTGAATCTCG13940.10773680069619304TruSeq Adapter, Index 8 (97% over 45bp)
AACTCGATTTGGAGCGATCCTGCCACTGCTTTATCAAGTAAGTTTATGTAATATTCTAAATTGTTCATTGTCATT13570.10487721559880485No Hit
AACTCGATTTGGAGCGATCGAAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCCGTCTTCTGC13570.10487721559880485TruSeq Adapter, Index 8 (98% over 60bp)
AACTCGATTTGGAGCGATCCTCCCACCTCAGCCTTCCAAAGGCACCCAGTCTTCTCTAAGAACTATTTTGGCATT13520.10449078518023887No Hit
AACTCGATTTGGAGCGATCAACATCCAGTAATGCTTGCTGAGTGAATGAATATGATAAATGAATTAGGAAATCTA13480.10418164084538609No Hit
AACTCGATTTGGAGCGATCCCTTCCAACTTAACCATTCCAAAGCTTAAGGGACTTCGGAGGTATTTCTATACAGA13450.10394978259424652No Hit
AACTCGATTTGGAGCGATCTCTGAAATCCCAGAATTTAAGTGGGCTGGAGGTTACGGGAAGCACCTTTATAATAT13360.10325420784082777No Hit
AACTCGATTTGGAGCGATCTCATGTTATCTTCCAATGAGTCCAACACAACCATCTGCCTTCCATTGCTGAGTCAG13200.10201763050141666No Hit
AACTCGATTTGGAGCGATCGTGCCACTGCACTCGAGCCTGGATGACAGAGCAAGACTCCATCTCAAAAAAAACCC13130.10147662791542429No Hit
AACTCGATTTGGAGCGATCACCTCAAAAAATATAGGAAGCACAGAATGACAACTATTCTGGTCTCAACTGACACA13040.10078105316200556No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)