FastQCFastQC Report
Sun 11 Jun 2023
EGAF00008066683

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008066683
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences874882
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACTCGATTTGGAGCGATCCCAAAGAGCAGCTTCCCTGAACTTTACTTTACTTGTCGTCGCTGCTGGATAGAGGC11141512.734860244010049No Hit
AACTCGATTTGGAGCAAGTGCAGTCCCAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCG401524.589418915922376TruSeq Adapter, Index 9 (100% over 45bp)
AACTCGATTTGGAGCGATCTTGAGCTGCGGACGGTTCAGGGGCTCCAGCTCGTTCCCTTGGATCTGATGCAGTAG104331.1925036747812847No Hit
AACTCGATTTGGAGCGATCTGATGCAGTAGGGCCATCCCAGATGCTTTGGTGTGGAGGGTCATGTCCCGCCGACA52890.6045386692148198No Hit
AACTCGATTTGGAGCGATCTGATGCAGTAGGGCCATCCCGGATGCTTTGGTGTGGAGGGTCATGTCCCGCCGACA47260.5401871338077592No Hit
AACTCGATTTGGAGCGAGTGCAGTCCCAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCG42870.49000893834825726TruSeq Adapter, Index 9 (100% over 45bp)
AACTCGATTTGGAGCGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCTTCTGC41070.46943473519857537TruSeq Adapter, Index 9 (100% over 60bp)
AACTCGATTTGGAGCGATCAGCCTGGATGACAGAGTGAGACCCTCATCTTTATGGATATATATGTATAAAGAACA31440.3593627483477772No Hit
AACTCGATTTGGAGCGATCGGAAAAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCTTCTGC29920.3419889767991569TruSeq Adapter, Index 9 (98% over 60bp)
AACTCGATTTGGAGCGATCCAGCAGGGTAGGCTTGTTTTGATTTCTCTCTCTGTAGCTTTAGCATTTTGAGAAAG27380.3129564901323835No Hit
AACTCGATTTGGAGCGATCGTGTAGAGCAAGTGCAGTCCCAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA27260.31158487658907147Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AACTCGATTTGGAGCGATCCACATAACCTCTTCCGCTAAACAAAGGCTTTCTTTTCTCCTTCTTCTACACATTCT22310.2550058179274462No Hit
AACTCGATTTGGAGCGATCTGCGTGGCTGTGGGATGCTCTAAATTTCTCCAAGGCGGCTGAAGATGCACACTGAA21860.24986226714002574No Hit
AACTCGATTTGGAGCGATCATGTCCCGCCGACACGCGAGCTCTGGCCCCGGCCCTGCCCCGGGAGCCTGCGGGTC20660.23614613170690446No Hit
AACTCGATTTGGAGCGATCTTACTTTTTTATTAATTTAAATTGTTACTGATTACGTGACACTTTGTGCTGGTCTA18030.20608493488264704No Hit
AACTCGATTTGGAGCGATCATCCAATCTCTGGCCTCAGTTCCACCTTTTTTGATTTAGCTAAAGTTTAGCTCACA15460.17670954483004564No Hit
AACTCGATTTGGAGCGATCACTCTGGTGATGTAACTCTTGTCTTAGCTCCACCTAGAGGGGGCATTGTGACGTAC15180.17350911322898402No Hit
AACTCGATTTGGAGCGATCTAATTATGTTACTGTAATCCTCAAGCTCGCATTTTTCAGCCACCTCAACACGAACT15060.1721374996856719No Hit
AACTCGATTTGGAGCGATCCAAATTAAATGAGTGTGAGAAAGATTAGAATAAGTGGCAATTATCCTTCAGGAGCT14840.16962287485626634No Hit
AACTCGATTTGGAGCGATCTCCCTCTTGCTACTGTATTTCTCTCTGTCCCAGTACCCAGTACAGAGGAACACCTG14630.1672225511554701No Hit
AACTCGATTTGGAGCGATCCACCTGCCTCAGCCTCCCAAAGTACTGGGATTATAGGTATGAGCCACCTCACCTGG14500.16573663648354864No Hit
AACTCGATTTGGAGCGATCGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCTTCTGCT14360.16413642068301781TruSeq Adapter, Index 9 (98% over 57bp)
AACTCGATTTGGAGCGATCACCAATCTGGGACATCAAGAAGCCAGACGACAAGGAGACATAGACCTCAGGTGAGT14160.16185039811083096No Hit
AACTCGATTTGGAGCGATCTGTGTGCCTCTGTGCTTAAGAGGAATGGCTGTATGACAGGGGTGGATAGCATTCCT14020.1602501823103001No Hit
AACTCGATTTGGAGCGATCACATTAATTTACCTCTTCCTGCCTTTATTTCCCTTTCTGTAAAATGGGAATTAATG13920.15910717102420668No Hit
AACTCGATTTGGAGCGATCACTGCAACGTTAAAGGGATATGTTCTATTAATATCCTATTTTACAGATGAGGAAAC13600.1554495349087077No Hit
AACTCGATTTGGAGCGATCTGTGCCCAGCAGCAGTTCATGTCCCGCCGACACGCGAGCTCTGGCCCCGGCCCTGC13050.14916297283519378No Hit
AACTCGATTTGGAGCGATCAGAGAAGCAAAGCTGTACTTTCCTAAGAAGGACCCCTGAGGAAGGTCTGTTCCTTC12860.14699125139161623No Hit
AACTCGATTTGGAGCGATCATCTTATAATTTAGATAGGCGGGGCAGAAATTTCTCACTATATAAGATGAGGAAAT12830.14664834800578821No Hit
AACTCGATTTGGAGCGATCAACTGAGGTCAGGAGTTTGAGACCAGCCTGGCCAACACAGCGAAACCCTGTCTCTA12580.1437908197905546No Hit
AACTCGATTTGGAGCGATCTAAAACTCCTGAAACCAAGTTTACTGGGAGACAACAGTCAAGACTACACTGTTAGT12460.1424192062472425No Hit
AACTCGATTTGGAGCGATCTGATGCAGTAGGGCCATCCCGGATGCTTTGGTGTGGAGGGTCATGCACCTCCTTTT12280.1403617859322743No Hit
AACTCGATTTGGAGCGATCCATGACTAGCGGCTGACTGATGCTGGGCCACAGAAGCTCAGCCACCTGTCGTAGCG12180.13921877464618088No Hit
AACTCGATTTGGAGCGATCTTCCTCAAATATGTTCTTTCCTCCTGAACCATAGTGATATTCCTAAGACACATTTC12080.13807576336008742No Hit
AACTCGATTTGGAGCGATCGTTCCCATGGCTTTCTTGATTCTATATAATCTTCTTTGATCTATTCTCTATTGCCT12030.1375042577170407No Hit
AACTCGATTTGGAGCGATCCAGGTTGTATTTCAGCAGTTCTGACAATTGCTCTGGGTCGAAGCTTGAATCAGTAG11790.13476103063041645No Hit
AACTCGATTTGGAGCGATCAGAAAGGAAGGGGGCTGAAATGTTATCTAAGTAAAAGGAAAAATGTATCGGAAACT11650.13316081482988562No Hit
AACTCGATTTGGAGCGATCTTAAGTATTTATGTTCAAACATACCAGGACACAGGAGAGTCCTAAGACTCAACTTT11520.13167490015796418No Hit
AACTCGATTTGGAGCGATCCCTCAAAACCTGAGCATACAGGACCTGTGGATATGGAGGACCAACTGTAAGGGACT11430.13064619000048006No Hit
AACTCGATTTGGAGCGATCTTATGGGACCACCATCATATATGTGTCCCATCATTGGCTGAAATGTCAGTATGTAG11310.12927457645716792No Hit
AACTCGATTTGGAGCAAGTGCAGTCCCAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATTTCG11230.12836016742829318TruSeq Adapter, Index 9 (97% over 45bp)
AACTCGATTTGGAGCGATCAGTCCCAGCAGATGGGCCTAGGTACAGGTCCCCACCTCCTCTCTTCCTGCTGCTCT11060.12641704824193434No Hit
AACTCGATTTGGAGCGATCCTTGCAACAAAATGAGTTCCTCTAAGGAGCTGAAAACAAAGCTCACTCCCCTCGTG11040.12618844598471565No Hit
AACTCGATTTGGAGCGATCCTGTATAAACAGTCTCCAGGCAGTCACAGTTTGGATGACAAGATCTTCAGGGTCTT11030.12607414485610632No Hit
AACTCGATTTGGAGCGATCCTGTATCTCTTATAATACTACTACTAATAACAATAACAACACTTGCTGAGTGTTTA10790.12333091776948206No Hit
AACTCGATTTGGAGCGATCCTTAGTACATGTCCCGCCGACACGCGAGCTCTGGCCCCGGCCCTGCCCCGGGAGCC10490.11990188391120174No Hit
AACTCGATTTGGAGCGATCAGATTGTTACTGTGTCTGTGTAGAAAGAAGTAGACATAGGAGACTCCATTTTGTTC10450.11944467939676437No Hit
AACTCGATTTGGAGCGATCTGTCACTATATCTTTTTTTTCTTTTATCACATTTAGAAACTGTCCTGAGAGGAGAT10400.11887317375371764No Hit
AACTCGATTTGGAGCGATCTTCTAGGCTCCACAAACTCTGGAAGGGCCATGTAGTTTTTTCTATTTGCTGGTAAG10340.11818736698206159No Hit
AACTCGATTTGGAGCGATCATTGAGGGAAAAAGGAACTATTTAAAAAGTCATGTGTGTGGTGTGTGTGTATATGT10250.11715865682457749No Hit
AACTCGATTTGGAGCGATCCCTTCCTTATACCTTATACAAAACTTAATTCAAGATGGATTAAAGACTTAAATGTC10110.11555844102404667No Hit
AACTCGATTTGGAGCGATCCCAAAGAGCAGCTTCCCTGAAAGCAACAGAAAAATCCTGTCAATTTCTCTGCATAA10060.11498693538099995No Hit
AACTCGATTTGGAGCGATCAGGTAGTATAATTCATTGCATGTTGATTATTCTGGCAAATATTTACCCTCCATTTT10060.11498693538099995No Hit
AACTCGATTTGGAGCGATCTATTTGTCTAATGGGACAAATTGACAAATTGGGAAAGATTCCATTACACAGGTGAC10050.11487263425239061No Hit
AACTCGATTTGGAGCGATCAATGACTGAGAGGTCCTGATGAGGTCAGAGCATTGTTGCAATGGGGCTATTCCAGT9960.11384392409490651No Hit
AACTCGATTTGGAGCGATCAACATCATCAGTGATAACCATAAATAACAAAGACAGACAAACAGTGATAAGTCCTA9880.11292951506603176No Hit
AACTCGATTTGGAGCGATCAGCCACCATGCCCTCATAATCTGACCTTCAAGTGTGGGTTTGGCTTATCGTCTTTT9850.11258661168020373No Hit
AACTCGATTTGGAGCGATCTGTCATCTGTGTAACAAGATGGACAAATTGCCAAATTCATCTAGAACAAGGCAAGA9770.11167220265132898No Hit
AACTCGATTTGGAGCGATCAGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCTTCTGC9740.11132929926550095TruSeq Adapter, Index 9 (98% over 60bp)
AACTCGATTTGGAGCGATCAATAATAGATATCTCCTAGGTCAAGTACATGCGGTGTTTGGTTTTTTGTTCTTGCG9710.1109863958796729No Hit
AACTCGATTTGGAGCGATCCACCCACCTCAGACTCCAAAATGCTGGAGATTACAGGTGTGAGCCACAACTCCTGG9700.11087209475106358No Hit
AACTCGATTTGGAGCGATCACCTGGCTTGACTGTAGTTATACTTTAGGCTCTGGCAGAACTACAGTCTATGAGCC9680.11064349249384489No Hit
AACTCGATTTGGAGCGATCTGGATTTCTTCTCGATTGCCTGTAGCATCAAATCTGTGACGAGGCTTACCCAGGAC9570.10938618007914211No Hit
AACTCGATTTGGAGCGATCCAAGCATCTCCCATTAGGCCCCACTTCCCAAGACTGTTGCATTGGGAATTAAGCTT9560.10927187895053275No Hit
AACTCGATTTGGAGCGATCTAGAACTAGAAATACCATTTGACCCAGCCATCCCATTACTGGGTATATACCCAAAG9470.10824316879304867No Hit
AACTCGATTTGGAGCGATCAGAGCATTCATTGCACTCCAGCCTGGGAAACAAGAGCAAAACTCAGTCTCCAAAAA9460.10812886766443933No Hit
AACTCGATTTGGAGCGATCAACTGAGGTCAGGAGTTCACGACCAGCCTGGCCAACTTGGTGAAACCCTGTCTCTA9340.10675725412112719No Hit
AACTCGATTTGGAGCGATCAAAGCACTGGGGTTAAGTTAAAACATAAGATGTTGTTTTTGGAAATACATGTCCCG9220.10538564057781506No Hit
AACTCGATTTGGAGCGATCCCAGCCCACTGCCTAGCACAAAGTAGGTGCTCAATACTCAATTCATGAATCATCGT9180.10492843606337769No Hit
AACTCGATTTGGAGCGATCCACAAATACCTTCCTAGTTTCTAATTATTCTACCGCTTAGGAGAGAGGGGTAAATG9110.10412832816311228No Hit
AACTCGATTTGGAGCGATCAACATCCAGTAATGCTTGCTGAGTGAATGAATATGATAAATGAATTAGGAAATCTA9040.10332822026284688No Hit
AACTCGATTTGGAGCGATCCCTTTCGAGTCCATTCAATGATTCCATTCCAGTCCATTCGATGATTCCATCTGATT9040.10332822026284688No Hit
AACTCGATTTGGAGCGATCAGAGTGTACAGTGAGTTAAAGAGAGAGTAAAAGGTACTCAACTCTGTCTTCAAGGA8940.10218520897675343No Hit
AACTCGATTTGGAGCGATCCTATTGGAATTGTTATTGCCTTAAAACCACTAGGATAGCAACCAAGTCTTGCATGA8880.10149940220509736No Hit
AACTCGATTTGGAGCGATCCAATAAGAGGATTGTATGACCTGCTTGTTGCCATGTTCAGCAACCCAGAAACTCTC8770.10024208979039458No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)