FastQCFastQC Report
Sun 11 Jun 2023
EGAF00008066950

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008066950
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6777
Sequences flagged as poor quality0
Sequence length77
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTTCATGATCTCGTATGC510.7525453740593183Illumina Paired End PCR Primer 2 (97% over 36bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAG120.17706949977866313Illumina Paired End PCR Primer 2 (96% over 33bp)
TCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTTCATGATCTCGTATG100.14755791648221928Illumina Paired End PCR Primer 2 (97% over 37bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTTCATGATCTCGTTTGG90.13280212483399734Illumina Paired End PCR Primer 2 (97% over 36bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTTCATGATCTCGTTTGC90.13280212483399734Illumina Paired End PCR Primer 2 (97% over 36bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTTCATGAGCTCGTATGC80.11804633318577543Illumina Paired End PCR Primer 2 (97% over 36bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)