FastQCFastQC Report
Sun 11 Jun 2023
EGAF00008067085

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008067085
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4300
Sequences flagged as poor quality0
Sequence length77
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAA110.25581395348837205Illumina Paired End PCR Primer 2 (96% over 30bp)
AGCTTCAAATGTCATTTTTAAAGGTTAACTGCAAAGAGCCTGTTGAATCT70.16279069767441862No Hit
AAAATCTGAAGTCATGTCATCTTTAAGAGACTTCATGGGGAGAGATGGGA50.11627906976744186No Hit
AATGATGAGTTAATGGGTGCAGCACACCAACATGGCACATGTATACATAT50.11627906976744186No Hit
TTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGA50.11627906976744186No Hit
TTTATTCCTATGTGCTTGATTGAAGCCAGCCTCTCTGCAACCAGAGTTGG50.11627906976744186No Hit
TAGAAATCATGGGTTCAATTTGGACATTTTGAAAAAAATTGTGTAGTCAT50.11627906976744186No Hit
TGACCGCGTGGACACCGATATCCCGGTCATCATCGCGCACGATACGGGCG50.11627906976744186No Hit
CGTAGTTTCCAGAGACTACGCATCGCCCTGGTGATTTCTGTGCCTGGAAT50.11627906976744186No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)