FastQCFastQC Report
Sun 11 Jun 2023
EGAF00008067312

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008067312
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2804
Sequences flagged as poor quality0
Sequence length77
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGAGACACATCTCGTATGC50.1783166904422254Illumina Paired End PCR Primer 2 (97% over 34bp)
AAGGCGCATTAGAGGTGATTGATTTAGTCCTTCAGCATCAACCTCCATTT40.14265335235378032No Hit
CCACATAGCTAACATCATACTTCATGATGAAAGACTGAATGCTTTCCCCT40.14265335235378032No Hit
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGAGACACATCTCGTTTGG40.14265335235378032Illumina Paired End PCR Primer 2 (97% over 34bp)
GGCCACTGAGAACTTGGCAAAGCTCGGGCGCCGCCGCCAGGCAGCCAGCA30.10699001426533523No Hit
TATTTTGTATCTGTTAATCCCAAACTCCTAACTTATCCCTTCCCCTTTGG30.10699001426533523No Hit
CGCTCCAACTGCGTTTCGCCGTTCGCCTGGACCCGCATGATCGGCACTAT30.10699001426533523No Hit
TCCCTCAGCAACCACAGGGGATTGGTTCCAGAAGACCCTGTGGATACCAA30.10699001426533523No Hit
TTCTCGTTGTGGTGCAGTGGTTAACGATCCGACTAGGAGCCATGAGGTTG30.10699001426533523No Hit
GATTTACTTCTCCCTTGATGTTCTGCGATCTTACTTGCTTACTGCATATA30.10699001426533523No Hit
GTGTACCCACACTGCCAAAATAATATTTGTATTTTTCTTGCTATTTTCAA30.10699001426533523No Hit
GGCTCCAGGTATTATGATGACTAAATTAACTAAAAAAATAGGCTGTTCTA30.10699001426533523No Hit
CCGCCGCAGTGCGCCCAGCATCCGCCACCTGTGCGGCAGCGGACATCTGC30.10699001426533523No Hit
CGAGATGGTGTCCATGAGCGAGCGCAGCCGCAAAATGACGTCGCACCAGC30.10699001426533523No Hit
GGAACTGCACCGGCGAGATGTTGACGGCGACGCGGATGTGGCTGGGCCAG30.10699001426533523No Hit
ATACATGATGATATTTGCGGATTGATTTGGTTATGACCTTGGCTTTGAAT30.10699001426533523No Hit
AAAGACATTTCCCCAAAGTAGAATAAAATGATATACAAGTGGAGGACAAG30.10699001426533523No Hit
TCAAACTGGTATTGAGCGACCGCTTCAAAGTTCTGTGTCTTATTGGCAAA30.10699001426533523No Hit
GAAGAGAGGACAAATCTTTGTTCTTCCTCTTCTTTACATACGCAAACCAC30.10699001426533523No Hit
ACGCCATTCGCCCGTGCCGTCGGCATTGAAGCGCGCGACATAGAGGGTGC30.10699001426533523No Hit
AACTACTCATGAATTAGTTTTTCATCTGGTATCCCAACATCACCAGACTC30.10699001426533523No Hit
AGCTTTGTTGTGGTTTTTCATTTTTAAATAAGCAATTTCGAAACGATACA30.10699001426533523No Hit
AAAAAATTAATAAGAAGAAAATTTGTCGCATATGATTTAGTGAATGTTAG30.10699001426533523No Hit
TTTTCAGACCCCATCCGCCTTCATCTTTATATGGCGTTCTCCCTGTGTGT30.10699001426533523No Hit
CATGGGATGCTCCATGGGCTCACATCGCTGCTCTCCGCCTGCTTCTCTTT30.10699001426533523No Hit
CTTAGAGGGCCAACTTAAAGAATTTTTTACGATTCAGCAATTGGCCGTTC30.10699001426533523No Hit
CTTACCACAGCTTGCATATCATAGTTCAATATTGAAGTTTTAAAAATATT30.10699001426533523No Hit
TAGTAGAATGTGTATTCTCTATAGTAGTGTTATATATCTTATAGGAAGTT30.10699001426533523No Hit
GATGCTGATGTTTCAGCCCAGAGCAAGAGTCACACAAGCCAAAGCTGAGG30.10699001426533523No Hit
CCCTTAAAAAAAATGATTGAGGAATCCAAACATCTAAAGAAAGAGTCCAT30.10699001426533523No Hit
CTAGAAATCCTGAAGATGCAAACGAATCAGTTCGCCAAGCAGGTTAACAC30.10699001426533523No Hit
AGGAGACCGCGCTGAGCTCGTCCTCGACGATGAAGCCGGTCAGGCCGTCC30.10699001426533523No Hit
CGCCGGCTCGCAAGCGAACTTGCGCCTGGGGTGCAGCCCGATGCCGGGCC30.10699001426533523No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)