FastQCFastQC Report
Sun 11 Jun 2023
EGAF00008067455

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008067455
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3756
Sequences flagged as poor quality0
Sequence length77
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCAATTCATCTCGTATGC190.5058572949946752Illumina Paired End PCR Primer 2 (96% over 33bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAG190.5058572949946752Illumina Paired End PCR Primer 2 (96% over 33bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCAATTCATCTCGTATGG120.3194888178913738Illumina Paired End PCR Primer 2 (96% over 33bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCAATTCATCTCGTTTGG100.26624068157614483Illumina Paired End PCR Primer 2 (96% over 33bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCAATTCATCTCGGTTGG80.21299254526091588Illumina Paired End PCR Primer 2 (96% over 33bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCAATTCATCTCGTTTGC70.1863684771033014Illumina Paired End PCR Primer 2 (96% over 33bp)
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAA70.1863684771033014Illumina Paired End PCR Primer 2 (96% over 30bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCAATTCATCGGGGGTGG60.1597444089456869Illumina Paired End PCR Primer 2 (96% over 33bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCAATTCATCGCGGTTGG60.1597444089456869Illumina Paired End PCR Primer 2 (96% over 33bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCAATTCATGGGGGGTGG60.1597444089456869Illumina Paired End PCR Primer 2 (96% over 33bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCAATTCATCGCGTTTGG50.13312034078807242Illumina Paired End PCR Primer 2 (96% over 33bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCAATTCATGGCGTTTGG50.13312034078807242Illumina Paired End PCR Primer 2 (96% over 33bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCAATTCATCGGGTTTGG40.10649627263045794Illumina Paired End PCR Primer 2 (96% over 33bp)
CTGCTCTAGATCATTCTTTATGGAAACTGGTGAACATTACAGAAACATTT40.10649627263045794No Hit
ATGTGCCGTGAGTGCAGTCCTGAAAAAGCAAAACCAAAACCAAAAGCAAA40.10649627263045794No Hit
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCAATTCATCTCGTGTGG40.10649627263045794Illumina Paired End PCR Primer 2 (96% over 33bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCAATTCATCGGGGTTGG40.10649627263045794Illumina Paired End PCR Primer 2 (96% over 33bp)
GAGTGTATACCCACCCTTTCAGAGCTTGGGCGACAGACCTCAGCTGGAAG40.10649627263045794No Hit
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCAATTCATGGCGGGTGG40.10649627263045794Illumina Paired End PCR Primer 2 (96% over 33bp)
CAAGCAATCTGCCCACCTTGGCCTCCCAAAGTGCTGGGATTACAGGCATG40.10649627263045794No Hit
CGGAAGAGCGGTTCAGCAGGAATGCCGAGGATCGGAAGAGCGGTTCAGCA40.10649627263045794Illumina Paired End Adapter 2 (100% over 29bp)
CCCTACTGGACAAGGCTCCCTCCGTCCTGGCCTGTGGGCGCAACACAACA40.10649627263045794No Hit
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCAATTCATGTCGTATGC40.10649627263045794Illumina Paired End PCR Primer 2 (96% over 33bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCAATTCATGTCGTATGG40.10649627263045794Illumina Paired End PCR Primer 2 (96% over 33bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)