FastQCFastQC Report
Sun 11 Jun 2023
EGAF00008067506

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008067506
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9098
Sequences flagged as poor quality0
Sequence length77
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGAGTAATATCTCGTATGC200.21982853374367994Illumina Paired End PCR Primer 2 (97% over 34bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGAGTAATATCGGGGGGGG170.18685425368212794Illumina Paired End PCR Primer 2 (97% over 34bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGAGTAATATCTCGTTTGG120.13189712024620795Illumina Paired End PCR Primer 2 (97% over 34bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGAGTAATATCTCGTATGG120.13189712024620795Illumina Paired End PCR Primer 2 (97% over 34bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGAGTAATATCTCGTGTGG120.13189712024620795Illumina Paired End PCR Primer 2 (97% over 34bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAG120.13189712024620795Illumina Paired End PCR Primer 2 (96% over 33bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGAGTAATATCGGGGGTGG110.12090569355902396Illumina Paired End PCR Primer 2 (97% over 34bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)