FastQCFastQC Report
Sun 11 Jun 2023
EGAF00008067521

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008067521
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1958
Sequences flagged as poor quality0
Sequence length77
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATTACCAGGTCCCAGGAGCAGCACTCGGGTGAGGACTGACATGCGTCA20.10214504596527069No Hit
AGGTCTTACATTTAAGTCTTTAATCCATTTTGAGTTGATCTTTGTCTATG20.10214504596527069No Hit
GAAGACCTGGGTGCTTTGGGACTGTGCCTGGAAGAAATCAGGACTCACAC20.10214504596527069No Hit
CTCTTCTCCAAAGCCCACCCTCAGCTTCCCTCTTCCCTAAGATCTTGTAG20.10214504596527069No Hit
GCCAGCACTGAAGTGCAAATCACATCATTCCCAGTCTCCCTGGAAGATGA20.10214504596527069No Hit
AAAACATGGGGAAACCTAAATGCATATTACCAGAGGAAAGAAGCCAATCT20.10214504596527069No Hit
AAAGAAAGAAAAATAGACATAAACTTGACCATCTTCACTGTCTTTAAGTG20.10214504596527069No Hit
GCTTTATGGTGAGTTAATTGACAAGGAGACAGGAGAAAACACTCAGATCT20.10214504596527069No Hit
AATAGTGATGGAGTCTGGACAATGAAAGGTGCAAACACATGAAGGGGAGG20.10214504596527069No Hit
AACTTTCAACTCTGAAGCTCAAGCAATCCTCCTGCCTCAGCCTCCCAGGT20.10214504596527069No Hit
CAGATAATAATTCTTTTATTTTTATTCTTTAAGTTCTGGGGTACATGTGC20.10214504596527069No Hit
TTGATGGCTCTGTGTAGACAATTCTAATTGGACTAAGGAGTTGACTGCAT20.10214504596527069No Hit
AGAGAATAGAATGGAATGCAAGCGAAAGGAAAGGAATGGATTGGAATGGA20.10214504596527069No Hit
GTTGAACCTTTCTTTTTACAGAGCAGCTTTGAAACCCTGTTTCTGTGGAA20.10214504596527069No Hit
TCAGTCTCCTGATCTGCACAACCTGCCTTGCCTGCAACGTTAACCATTCT20.10214504596527069No Hit
CCCTGGTGCACGCATGCAATCACTGTACAGGGTCATCTGTGTGCAAACAC20.10214504596527069No Hit
CTCATCGAGCACAGGGGCTAATCATGGCTGAACCTTAAAACAGGTAGGAG20.10214504596527069No Hit
AACAAATATCACTCTAACATTCATTCCATGGCTACTTCACCAACATGCTG20.10214504596527069No Hit
ACCATTCCCTCGGTGGTGTCCTCACAACAGTGAGTTCACATGGGAGCTGG20.10214504596527069No Hit
AATGGAATGGAATGGAATGGAATGGAACAGTCAATGAACTCGAATGGAAT20.10214504596527069No Hit
GAAAACTCATCCTAAGAGTCATATGGAATCTCAAGGGACCCCAAATAGCC20.10214504596527069No Hit
TTGCAGCAGGCTAGTCCCTCAGACATGTCCCAGGCATCATGTGGACAGCA20.10214504596527069No Hit
ACTTGACGACTTGAAAAATGACGAAATCACTAAAAAACGTGAAAAATGAG20.10214504596527069No Hit
ACTGAAGACAAAAAACATGATTATCTCAATAGATGCAGAGAAAGCTTTTG20.10214504596527069No Hit
CAGGTCGTCTGCCTCCAAAGCCCACCCTGTTTCCATCATGCCAAGAGGTG20.10214504596527069No Hit
TCCGCTGACCCTTAATGAGTTAGAGATATTATGTAAATGGAGATCAAATT20.10214504596527069No Hit
GCAGAGTAAGGATGTGTGTGTCTAGGCATGTGGGGGTGTGGGTGTGACGG20.10214504596527069No Hit
CTCATATTAGAAACAGTAACAAAACTAGATTAAATTTGTAAAAGTGCATT20.10214504596527069No Hit
AATGATCTTTCTCTTTTTCCTCCATGTATTTGCCTCCTCTTGATCAGTCA20.10214504596527069No Hit
CATACCCATTTATCAGTAAATGTGACTGGTGAACTGTATGCCCTAGAATG20.10214504596527069No Hit
ATGACCCATAGGATGAAATGCGATCTGCTCAGCCAGGCACACGAGGCCCT20.10214504596527069No Hit
CTGCCCCGCGGTGCTATGCAAGGAGCAAACCCAGACTCTAGTTTGAGTTC20.10214504596527069No Hit
TCGGCCAAGCACCTCAGCCATCCTCTCTATTGATGTGTCTGAGTTCTTAT20.10214504596527069No Hit
AGCGTTCTGAGAAACTGCTTTCTGATGTTTGCATTCAAGTCAAAAGTTGA20.10214504596527069No Hit
GGTACACTGAGAACACATGGACATGTGGGAAAGAACATACTGGGGCCTGT20.10214504596527069No Hit
GATTTGGCTGGTCATCGCTTTCTCCCTGGACCTGCTTATATACGCAGGTG20.10214504596527069No Hit
TAGAGGTTAACAGCAAACACAATACAGGCACACCTCACAGAAACCATAGG20.10214504596527069No Hit
TGCCACCAGGCATGACAACGCTCCTTCAGCAACCAACTAAACGAGCACAA20.10214504596527069No Hit
TGCCAGCTACTTGGGAGGCTGAGGCAGGAGAATTGCTTGAACCCGGGAGG20.10214504596527069No Hit
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATAGATATCTCGTGTGG20.10214504596527069Illumina Paired End PCR Primer 2 (97% over 35bp)
AGTGAGATCCAGTCGAAAATAAGGAATGGAATGGAATGGAGTAAAATGGA20.10214504596527069No Hit
CCAGGCTGGTCTCGAACTCCTGACCTCAAGTGATCCGCCCTCCTCAGGCT20.10214504596527069No Hit
GTTAGGAACTGGTGTTCCCTTCTCCTGCTCTGGAAGTCAAGCAACCACCC20.10214504596527069No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)