FastQCFastQC Report
Sun 11 Jun 2023
EGAF00008067610

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008067610
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4778
Sequences flagged as poor quality0
Sequence length77
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGAGTGGTATGGGGGGGGG501.0464629552113855Illumina Paired End PCR Primer 2 (97% over 34bp)
AAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAA450.9418166596902469Illumina Paired End PCR Primer 2 (96% over 30bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGAGTGGTATCGGGGGTGG420.8790288823775638Illumina Paired End PCR Primer 2 (97% over 34bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGAGTGGTGTGGGGGGGGG360.7534533277521975Illumina Paired End PCR Primer 2 (97% over 34bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGAGTGGTATCGGGGGGGG280.5860192549183758Illumina Paired End PCR Primer 2 (97% over 34bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGAGTGGTATGGGGGGTGG240.5023022185014651Illumina Paired End PCR Primer 2 (97% over 34bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGAGTGGTGGGGGGGGGGG180.37672666387609877Illumina Paired End PCR Primer 2 (97% over 34bp)
TCTTTTTACCTGCAAGGGTACATGTGTGGATTTGTTAGACGGTTATATTG80.1674340728338217No Hit
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGAGTGGTATCGGGGTTGG80.1674340728338217Illumina Paired End PCR Primer 2 (97% over 34bp)
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGAGTGGTTTGGGGGGGGG80.1674340728338217Illumina Paired End PCR Primer 2 (97% over 34bp)
TGGTTAAATGAATTACTATTATTTAATTTTATTATTTAATTAACAGTGAC70.14650481372959395No Hit
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGAGTGGTATCGGGGGTTG60.12557555462536626Illumina Paired End PCR Primer 2 (97% over 34bp)
ACCACTCTGTCATCCCGCAAATAAGCTCAATATCATGCATTGATTTTCTT60.12557555462536626No Hit
CCCTTCTTAAGAAGCTAAGTCCATATCCCAACCACTTCCTTTATGAGACT60.12557555462536626No Hit
AACAGGGTAAAAAAGGACTGTTTTTAGGTTGTACAGCATATCCCGCCTGT50.10464629552113855No Hit
GAAGTAGATTTTGACACAAAGCGTCACGCTGAGGGTGCAGAGCTATGTGA50.10464629552113855No Hit
CGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGAGTGGTAGGGGGGGGGG50.10464629552113855Illumina Paired End PCR Primer 2 (97% over 34bp)
CACTTAAGTGAAGGGTATGAGCTGATCTGGATCGTTCGATCCACTTTAAA50.10464629552113855No Hit
TGGAACGACAGTGCCCATATCTTACTTACTATGTACTGCCATACACGAAG50.10464629552113855No Hit
GAAACACTCTTTTTGTAGAAACTGTAAGTGGATATTTGGATAGCTCTAAC50.10464629552113855No Hit
GAAAAAAAAAAGAATATATGTAGATTCCTACAATAAAAAATAATCAGACA50.10464629552113855No Hit
CAACAAGTATTTGTCACAGCAGACTACTCCCTGAACCTATTAGTCCAGCA50.10464629552113855No Hit
TGTTCATTTTTGTTTTTACTGTCATTACTCTAAGAGGTGAATCTGAGAAG50.10464629552113855No Hit
GTTTTGCAGGAGGTGATGGAAGGTCACGCCCGGGCCAGGGTGGTCTGAGG50.10464629552113855No Hit
AATCAGGTCTTACATGTAGTAGGCACTCAATTAACTGAATTGCTCTTGGA50.10464629552113855No Hit
ATATAAGTTGAGGTTATCAATATGCTGCTGATAACGCTGTTCTTGGCGCG50.10464629552113855No Hit
GTACATAACGAAATGAAGGCAGAAATAAAGATGTTCTTTGAAACCAACAA50.10464629552113855No Hit
TTATGTCATCTACAAATAAATATTTACTCCCTTCCATTTTGGCTGCTATT50.10464629552113855No Hit
CAGAGCCAGAAAACAAATAAAAAAAAGAGAGAGAGAGAGATAGCAGCCTT50.10464629552113855No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)