FastQCFastQC Report
Sun 11 Jun 2023
EGAF00008071090

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008071090
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3633092
Sequences flagged as poor quality0
Sequence length121
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTATG125330.34496786759047116TruSeq Adapter, Index 27 (97% over 38bp)
AAGGCATGGGGTCTGGAGAAAAAACAGAATCATCTCCTGAAGAACAAGAA53440.14709233897737795No Hit
CAGGAATACTTGGACCTCAGCCAACCTCTCGAACAGTATTCACCTAGTTA52490.14447748639450914No Hit
ACTCACCATCCTGTGTGCAGGCTCCAAGAAGATTTATGATATTCTTGTGT45630.12559549826979333No Hit
CAGGTACGAAACCAGCACTGGAGCCTCATTATGGAAAGTGTGGTCCCATC44850.1234485666754379No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGAACTCGTATG44240.12176955606959582TruSeq Adapter, Index 27 (97% over 38bp)
CCACTGGATGTGGGGCTGGGCATCACTGTAAACCTTGCAGACAAACTCTA42270.11634717755564682No Hit
CCACCAGAGCGATCGCCTCACCGGCCCATCCTCCAAGCCGGACTGCCGGC38840.10690618349328891No Hit
CCAGGTGGTACGTGTGATTGATGGACCCGTATTCATTCTCCACCACACAG38270.10533727194356762No Hit
GAGGATACCACATTAGAGCCAGAAGGTAAGTCATTTAATTTCACTTTTCA38250.10528222241550722No Hit
GTGCACAGCCGGCTGGCTGCTGAAGTCTGGCTTCTTGGTCGTGTTCTTCA38200.10514459859535626No Hit
GTTCATAGATGATGCCACAGAGAAAGACCTTTCTGATCTGGTGTCAGAGA36940.10167647832755129No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)