FastQCFastQC Report
Sun 11 Jun 2023
EGAF00008071091

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008071091
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3633092
Sequences flagged as poor quality0
Sequence length121
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATCTC152890.4208261172576967Illumina Single End PCR Primer 1 (96% over 33bp)
AAGGCATGGGGTCTGGAGAAAAAACAGAATCATCTCCTGAAGAACAAGAA56830.15642323398361505No Hit
CAGGTACGAAACCAGCACTGGAGCCTCATTATGGAAAGTGTGGTCCCATC47840.13167847112046707No Hit
CAGGAATACTTGGACCTCAGCCAACCTCTCGAACAGTATTCACCTAGTTA46150.12702678599936362No Hit
ACTCACCATCCTGTGTGCAGGCTCCAAGAAGATTTATGATATTCTTGTGT43470.11965014923927059No Hit
CCACCAGAGCGATCGCCTCACCGGCCCATCCTCCAAGCCGGACTGCCGGC40270.11084222474960723No Hit
GAGGATACCACATTAGAGCCAGAAGGTAAGTCATTTAATTTCACTTTTCA39210.10792459976240623No Hit
CCACTGGATGTGGGGCTGGGCATCACTGTAAACCTTGCAGACAAACTCTA39180.10784202547031564No Hit
GTGCACAGCCGGCTGGCTGCTGAAGTCTGGCTTCTTGGTCGTGTTCTTCA37930.1044014299665409No Hit
AGACAGTGAAACTTGGTACTTCATGGTGAATGTCACAGGTGAGTTGGCCC36730.10109845828291714No Hit
GTTCATAGATGATGCCACAGAGAAAGACCTTTCTGATCTGGTGTCAGAGA36640.10085073540664535No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)