FastQCFastQC Report
Sun 11 Jun 2023
EGAF00008071092

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008071092
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9406481
Sequences flagged as poor quality0
Sequence length0-121
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[FAIL]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGATCTCGTATG206240.21925308731288565TruSeq Adapter, Index 1 (97% over 35bp)
GCTTGAAAAATATTGATTGTTTCTAATAGAGCAGCCAGAACTCTTTATTT176680.18782794543464235No Hit
AGGGTCTGCACATGTTACAATCAAAACCAATGTCTGCTACATTTTCCACT167550.17812187150540143No Hit
GCTTGGCTTCACCAGTATGTGCGTCCCAAATAATTGTAGTCTGAGATTAA159200.16924501309256884No Hit
CCTCCCCATTTGCTGTGTTTTCTCCATTTTTTGCAGATCCTTGTTGGCTG159170.1692131201880916No Hit
ACAAACAAGACAACAAGCTTATAGAGATAAGCTTGCACAGCAACAGGCAG156720.16660853298911676No Hit
AATACAGTTAGGCATATACTTTACACATGTATGTTAAACTTCTTTCCATG156230.16608761554932178No Hit
CATGAAATACTCCAAAGCCTCTTGCTCAGTTTTATCTAAGGCTAGGGTCT155890.16572616263191303No Hit
GCAGATAATCATACTGATATGATGGAAGTGGATGGGGATGTTGAAATCCC148600.15797618684394302No Hit
CATAAATCTTTTCTCAATGATGCTTGGCTCTGGAATGCCAGAACTACAAT148320.15767851973548874No Hit
CAGAATACTGAATAAGCAAGCAAGACAGCTGACTTAACGGCATATTTAAG144370.15347928731265179No Hit
GTTCTTAATTTATTCAAGACATTTTGTATCTGCATATATCAAACTATAAC144110.15320288214051567No Hit
ATATTGCATACTTTTATAGGTCTGGAGACTCAACAGCAAGAATATGGAAT142840.1518527491843124No Hit
AACCAAATTTTTTCTTCTTGTGATCCAAAAAGTGTCCAAAATCTATATGA139290.14807875548783864No Hit
AACAGGGTTCCAGGCACAGATAAAAACTTCAGATTCATGGCCCCGCAACA139190.14797244580624783No Hit
ATACAATGTCTAAGTACTAACTGTGTAGAGCCACTGGTGCTGTTCTCACT132090.14042445841330037No Hit
ACAGTATGTAGAAGCAGAAGTTAGTATTAATGAGGTAAGGAAGACTTATT125810.13374821040939752No Hit
GTTTAAATGGAAACTTGCACCCTGTTTTCTTTTCTCAAGTTGGCCTGAAT122940.1306971225477413No Hit
AAAGGAGCCCAAGAATGCACAAAGACAAGAGAATTTGAGAGGTGAGCTCG120740.12835830955274347No Hit
GAACGTGTAAACAGGTCAATGGCTGCATCATATCTACAAAGAATAAACAC119250.1267742952970404No Hit
CACACACCTTTTGTGTTTCATCCTTCTTCTCCTGTTTGAGAATGTCAGTT119120.12663609271097237No Hit
ATTTGAAGCACCTGAATAGGCAAGTCGAGGCAATGGAAAAGCTCATTAAC116300.12363815969011152No Hit
GAGCCACTGATGTAGTGTGTGGCTGTTGAACTGCAGTGCACCTTTCAAGC107110.11386829995191612No Hit
CAGTGATTGAAGAGCATGCCAATTGGTCTGTATCCCGAGAAGCAGGATTT101910.10834019650919403No Hit
CAATCGGTGACTGTGTGGGACTTATTGAGGTGGTGCGAAATTCTCACACT100500.10684122999876362No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGAACTCGTATG99600.10588444286444632TruSeq Adapter, Index 1 (97% over 35bp)
ACAGATCACTAAAGTGGTTCTTAGCAAAGGTTGGAGGTGTCTTGAGTGCA98470.1046831434624702No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)