FastQCFastQC Report
Sun 11 Jun 2023
EGAF00008071093

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008071093
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9406481
Sequences flagged as poor quality0
Sequence length0-121
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[FAIL]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTTGAAAAATATTGATTGTTTCTAATAGAGCAGCCAGAACTCTTTATTT180590.19198465398484302No Hit
GCTTGGCTTCACCAGTATGTGCGTCCCAAATAATTGTAGTCTGAGATTAA171090.18188523423371608No Hit
ACAAACAAGACAACAAGCTTATAGAGATAAGCTTGCACAGCAACAGGCAG157880.16784172529557015No Hit
GCAGATAATCATACTGATATGATGGAAGTGGATGGGGATGTTGAAATCCC156450.16632149684882158No Hit
CCTCCCCATTTGCTGTGTTTTCTCCATTTTTTGCAGATCCTTGTTGGCTG156440.1663108658806625No Hit
CATAAATCTTTTCTCAATGATGCTTGGCTCTGGAATGCCAGAACTACAAT148380.15774230554444324No Hit
CATGAAATACTCCAAAGCCTCTTGCTCAGTTTTATCTAAGGCTAGGGTCT145560.15474437252358242No Hit
AACCAAATTTTTTCTTCTTGTGATCCAAAAAGTGTCCAAAATCTATATGA144670.15379821635742422No Hit
ATATTGCATACTTTTATAGGTCTGGAGACTCAACAGCAAGAATATGGAAT144110.15320288214051567No Hit
ACAGTATGTAGAAGCAGAAGTTAGTATTAATGAGGTAAGGAAGACTTATT141260.1501730562151776No Hit
AATACAGTTAGGCATATACTTTACACATGTATGTTAAACTTCTTTCCATG141010.14990728201120057No Hit
GTTCTTAATTTATTCAAGACATTTTGTATCTGCATATATCAAACTATAAC135780.14434728566400123No Hit
AACAGGGTTCCAGGCACAGATAAAAACTTCAGATTCATGGCCCCGCAACA129320.13747968023323495No Hit
GAACGTGTAAACAGGTCAATGGCTGCATCATATCTACAAAGAATAAACAC127890.13595945178648636No Hit
CAGAATACTGAATAAGCAAGCAAGACAGCTGACTTAACGGCATATTTAAG126080.1340352465496927No Hit
AGGGTCTGCACATGTTACAATCAAAACCAATGTCTGCTACATTTTCCACT124480.13233429164423977No Hit
CACACACCTTTTGTGTTTCATCCTTCTTCTCCTGTTTGAGAATGTCAGTT122200.12991043090396928No Hit
ATACAATGTCTAAGTACTAACTGTGTAGAGCCACTGGTGCTGTTCTCACT119200.126721140456245No Hit
ATTTGAAGCACCTGAATAGGCAAGTCGAGGCAATGGAAAAGCTCATTAAC109910.11684497103645879No Hit
GTTTAAATGGAAACTTGCACCCTGTTTTCTTTTCTCAAGTTGGCCTGAAT107930.11474003934096076No Hit
AAAGGAGCCCAAGAATGCACAAAGACAAGAGAATTTGAGAGGTGAGCTCG107460.11424038383748396No Hit
ACCTAAATCCACAGATTATAATTGATACTTAATAAACTCAGTGATTTGCC103700.11024313980966952No Hit
GAGCCACTGATGTAGTGTGTGGCTGTTGAACTGCAGTGCACCTTTCAAGC102710.10919067396192052No Hit
ACTTGAATATTAAACATACATACTTTAAAACTATTCTGAAACATTACTTG102200.10864849458580739No Hit
CAATCGGTGACTGTGTGGGACTTATTGAGGTGGTGCGAAATTCTCACACT101320.10771296938780826No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)