FastQCFastQC Report
Sun 11 Jun 2023
EGAF00008071094

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008071094
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61435376
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCACGCAATCTCGTAT10754811.750589106836426TruSeq Adapter, Index 1 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCACGCAATCTCGTTT4421010.7196195885575764TruSeq Adapter, Index 1 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCACGCAATCTCGGAT3789150.6167700511835396TruSeq Adapter, Index 1 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCACGCAATCGCGTAT3459190.5630615819784354TruSeq Adapter, Index 1 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCACGCAATCTCGGTT1698850.27652634534213644TruSeq Adapter, Index 1 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCACGCAATCGCGTTT1399400.22778407020736716TruSeq Adapter, Index 1 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCACGCAATCGCGGAT1220120.19860218646663771TruSeq Adapter, Index 1 (97% over 39bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)