FastQCFastQC Report
Thu 29 Jun 2023
EGAF00008071368

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008071368
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48856900
Sequences flagged as poor quality0
Sequence length101
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACACACACACACACACACACACACACACACACACACACACACACACACA3869820.7920723582544124No Hit
ACACACACACACACACACACACACACACACACACACACACACACACACAC1524810.3120971653952666No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT983450.20129193624646674No Hit
CTTGTTACGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTC748520.1532066095065385No Hit
ACGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCT626340.12819888285994402No Hit
GCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGTTGGGGG548580.11228301427229316No Hit
GTCAAATTAAGCCGCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTC540640.11065785999521051No Hit
CTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCTCCGC533660.10922919792291366No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTCACGAATCTCGGTT491630.10062652358213475TruSeq Adapter, Index 9 (97% over 38bp)
TGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTG490140.1003215513059568No Hit
GTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTT489870.10026628787336077No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)