FastQCFastQC Report
Thu 29 Jun 2023
EGAF00008071392

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008071392
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32279516
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAAGGTCAATCTCGTAT19913486.169076388877702TruSeq Adapter, Index 3 (97% over 38bp)
CACACACACACACACACACACACACACACACACACACACACACACACACA2432980.7537225775008523No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAAGGTCAATCGCGTAT2390560.7405811165198388TruSeq Adapter, Index 3 (97% over 38bp)
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT1208340.3743364677462946No Hit
TGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTG753360.23338639897822508No Hit
ACACACACACACACACACACACACACACACACACACACACACACACACAC524920.16261706030536516No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAAGGTCAATCTCGGAT469430.14542659189809415TruSeq Adapter, Index 3 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAAGGTCAATCTCGTTT394680.1222694912773785TruSeq Adapter, Index 3 (97% over 38bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)