FastQCFastQC Report
Sun 11 Jun 2023
EGAF00008071393

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008071393
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences145508547
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGCGAGACATCTCGTAT108423287.451334113040109TruSeq Adapter, Index 11 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGCGAGACATCGCGTAT13397960.9207679051320607TruSeq Adapter, Index 11 (97% over 36bp)
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT4171450.28668075422401135No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACA3959440.27211047609457606No Hit
TGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTG1655930.11380293695050094No Hit
GCCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGGAAAGGCCCAGA1615630.11103334019272422No Hit
GTCGTTTCCCCCTGGACTCTGCGCCCCTCACCGCCACCACACCGCACGGG1485840.10211358924503589No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)