FastQCFastQC Report
Sun 11 Jun 2023
EGAF00008074351

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008074351
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41157021
Sequences flagged as poor quality0
Sequence length75
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG5502761.3370161071667457TruSeq Adapter, Index 5 (100% over 63bp)
CGGGCGTAGTGGCGGGCGTTTGTAGTTTTAGTTATTTGGGAGGTTGAGGTAGGAGAATGGCGTGAATTTGAGATC607990.14772449152721717No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAG526910.12802432906890904TruSeq Adapter, Index 5 (100% over 63bp)
CGGGTTTACGTTATTTTTTTGTTTTAGTTTTTTAAGTAGTTGGGATTATAGGCGTTCGTTATTACGTTTGAGATC446850.10857199795874439No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)