FastQCFastQC Report
Wed 28 Jun 2023
EGAF00008082188

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008082188
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3562
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN165546.46266142616508No Hit
CCGCCTCTGTGCCGTTCATACACATCTCCGAGCCCACGAGACAAGAGGCA1454.070746771476698No Hit
CCTTACTGCCTCTTGCTTCTCTTTTCCTATCCAACGTTAACC1363.8180797304884897No Hit
ATCTCCATTTTAGCACTTACCTGTGACTCCATAGAAGACAAT1002.807411566535654No Hit
GCTAGGACTCCTGCTTCTGTCATATCATCCGATCCAA822.3020774845592364No Hit
GCATTTGCATTATGAAACCAATATTATGGATCCCAA621.7405951712521055No Hit
GGCTACATGTTCCTGATCTCCTTAGACAACTACTAAG601.6844469399213924No Hit
ACACAGTGAGACAAAAAGAAAGAATGAATCAAAGGGTGAA371.0387422796181922No Hit
CTCACATCTCCTCGCTGCAGTTGTCTCTTATACACATCCCCGAGCCCACG340.9545199326221224No Hit
CTTCCCTCATTTCCAACATAGGAGGATCCAATCTCCGGTCCTGT340.9545199326221224No Hit
GCCATCAGCACTGGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA260.7299270072992701Illumina PCR Primer Index 3 (96% over 27bp)
CCTTCACCTCCACATGCTTCAATTCCAATTTTACACCAGCA210.5895564289724873No Hit
ATGAAAGTAAAAGAGGAGAAACTCAGAGATAACCAATACATGGCATGGC200.5614823133071308No Hit
TAAATACACAACTGTAATTGAATTTATGGTGATTTGGT160.4491858506457047No Hit
CCTTCCTCCCTCTTTCTTTCATAAAACCTCTCTTATACACATCTCCGAGC150.4211117349803481No Hit
CCCATGAGCGTGCCCCGTAACATCTGCTCTTATACACATCTCCGAGCCCA130.36496350364963503No Hit
GCCATCAGCACTGGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAC120.3368893879842785Illumina PCR Primer Index 3 (96% over 27bp)
GCAGGAATGTAACAAGAGTTTTGAGGGCTCTAATAGCCCTCCCAGC120.3368893879842785No Hit
AACACAGCTTATTTATTGGTCTCTCATTCCTCCCAT120.3368893879842785No Hit
CCGCCTCTGTGCCGTTATACACATCTCCGAGCCCACGAGACAAGAGGCAA110.308815272318922No Hit
AACACAGCTTATTTATTGGTCTCTCATTCTCCCATCCGAG100.2807411566535654No Hit
TGTGAAACACAAACGATTTTACCACTGGCTATCCTAAATGCAGGTGGCCC80.22459292532285235No Hit
CTGTATTCCACTTTTGAATGTTGTACTGGGTGACATGCTGCAGCCAAGAC60.16844469399213924No Hit
CTGTGCCAGGGACCTTACCTTATACACCATCTCCGAGCCCACGAGACAAG60.16844469399213924No Hit
CCCTGGATTCTCTCTTTAGGGAGCTTCTCTTATACACATCTCCGAGCCCA60.16844469399213924No Hit
CCCTTGGAGATATCGATCTGTTAGAAACCTCTCCAATCCACCATAAATC60.16844469399213924No Hit
CCGCCTCTGTGCCGTTCATCACATCTCCGAGCCCACGAGACAAGAGGCAA60.16844469399213924No Hit
AATATGTGGGTTTGCAATTTATAAAGCAGCTTTTCCACTTATTTTCTTAG60.16844469399213924No Hit
TTCAGAACAGCTTCAAATAAGGAAATCAAGCTCTCTGAACATAACATTAA60.16844469399213924No Hit
CAACTTGTTACACAAATCAGTCCCCTTATTCAGTCATTGAAAATTCAGCC50.1403705783267827No Hit
GCCATCAGCACTGGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAT50.1403705783267827Illumina PCR Primer Index 3 (96% over 27bp)
AAAAAGACAGAGGTACCTGAATCAGCATTTGCAAATGTAAGTGGTGCTTC50.1403705783267827No Hit
TCTCAAAGGGCTTCTGATTTGCTACATTTGAATCTAATGGATCAGTATCA50.1403705783267827No Hit
CCTTGATAAACCTTGTTCCTTTTGTTCAGCAGATTCCATGGCCTTCCTAA50.1403705783267827No Hit
CTCTGAGAGACATCCCAGCCGAAGAATCCAGCCCTTGTCCCAAACGTGTG50.1403705783267827No Hit
ACCCCGTCTGCTGCCCTTACAGACAACGCCTCTGACAAGGACCTGGCCGA50.1403705783267827No Hit
CGCACCTTCATCTCGGCCAGATACTGTTTCCTATACACATCTCCGAGCCC40.11229646266142618No Hit
GCATCCCTGTGTAAGTGCATTTTGGTCTTCTGTTTTGCAGACTTATTTAC40.11229646266142618No Hit
GTGGCCCTCTTTTGGACTAGCAGAAAACACAGAAAAATCTCCAGCAAATA40.11229646266142618No Hit
AACAGTACCCGTTCCCTTGATGTCTACAATTTCACCTTTCTTACAGATTC40.11229646266142618No Hit
ACAAACAGTATCATTTTTACTTGAATCACTGCCATCAAATTCTAAGTTAT40.11229646266142618No Hit
TTGATAAGAGAAACCCAGAGCACTGTGTAAACTCAGAAATGGAAAAAACC40.11229646266142618No Hit
ACTTGACCATTCTGCTCCGTTTGGTTAGTTCCCTGATTTATCATTTCAGG40.11229646266142618No Hit
GAGGCCAGTCACAATGGACAGCGCCGGGGCCTCCCCAAGGCCGTGTGTAT40.11229646266142618No Hit
TAACCCTGAGCCAAATGTGTATGGGTGAAAGGGCTAGGACTCCTGCTAAG40.11229646266142618No Hit
TGAAAATGCCAGCACTCTTATTTTAACTCCTACTTCCAAGGATGTTCTGT40.11229646266142618No Hit
AGGGATGAGCTTGACTTGCCGGAAGAGCCTGACTCCAGGGAGAACTGCAA40.11229646266142618No Hit
GCCTCTGAACTGAGATGATAGACAAAACCTAGAGCCTCCTTTGATACTAC40.11229646266142618No Hit
CTGTGAGCAAACAGTATGTATTTCCAGTCCACTTTCAGAGGCTTCAGTTT40.11229646266142618No Hit
GACTTACCATGACTTGCAGCTTCTCTTTGATTTGTGTTTTCACTGTCTGT40.11229646266142618No Hit
CTCACATCTCCTCGCTGCAGTTGCCTCTTATACACATCTCCGAGCCCACG40.11229646266142618No Hit
GCCAACAATTGCTTGACTGTTCTTTACCATACTGTTTAGCAGGAAACCAG40.11229646266142618No Hit
TAGTAACACTCAGCTGAATTAAGGCGGTTTGTCCCGTCAAAGCCCCCCAC40.11229646266142618No Hit
GAGTTTCCTTTCTTGCATCTTAAAATTCATCTAACACATCTCCGAGCCCA40.11229646266142618No Hit
GAGTCAGCTCGTGTTGGCAACATACCATCTTCAACCTCTGCATTGAAAGT40.11229646266142618No Hit
GCTAGGACTCCAATCCCTGTCCATTCCTTCTCTTATACACATCTCCGAGC40.11229646266142618No Hit
TAAAAAGCTAATTTTACCATCTGGAGTGCTTTTTGAAGCCTTTAAGGATT40.11229646266142618No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)