FastQCFastQC Report
Wed 28 Jun 2023
EGAF00008082189

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008082189
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3562
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGCAGCGAGGAGTTGTGAGCAAAACGCAATACTGTACATT481.347557551937114No Hit
ACAAACTGGTCAGTTGTGAGACCAAAACGCAATACTGTACATT461.291409320606401No Hit
ACAAACTGGTCAGTNGTGAGACCAAAACGCAATACTGTACATT350.9825940482874789No Hit
CTGCAGCGAGGAGATGTGAGCAAAACGCAATACTGTACATT330.9264458169567659No Hit
CTGCAGCGAGGAGNTGTGAGCAAAACGCAATACTGTACATT300.8422234699606962No Hit
ACAAACTGGTCAGTGGTGAGACCAAAACGCAATACTGTACATT290.8141493542953397No Hit
GGTTAACGTTGGATTGGAAAAGAGAAGCAAGAGGCAGTAAGGCAAAACGCAATACTGTACATT280.7860752386299831No Hit
CTGCAGCGAGGAGANGTGAGCAAAACGCAATACTGTACATT220.617630544637844No Hit
ACAAACTGGTCAGTGNTGAGACCAAAACGCAATACTGTACATT140.39303761931499154No Hit
TTGGATCGGATGATATGACAGAAGCAGGAGTCCTAGCCAAAACGCAATACTGTACATT140.39303761931499154No Hit
ACAGGACCGGAGATTGGATCCTCCTATGTTGGAAATGAGGGAAGCAAAACGCAATACTGTACATT140.39303761931499154No Hit
GGTTAACGTTGGATAGGAAAAGAGAAGCAAGAGGCAGTAAGGCAAAACGCAATACTGTACATT140.39303761931499154No Hit
ACAAACTGGTCAGNTGTGAGACCAAAACGCAATACTGTACATT130.36496350364963503No Hit
GGTTAACGTTGGANAGGAAAAGAGAAGCAAGAGGCAGTAAGGCAAAACGCAATACTGTACATT120.3368893879842785No Hit
ACAAACTGGTCAGNGGTGAGACCAAAACGCAATACTGTACATT110.308815272318922No Hit
TTGGATCGGATGATTGGACAGAAGCAGGAGTCCTAGCCAAAACGCAATACTGTACATT110.308815272318922No Hit
AGGGGAGGGACATTTGATGGACCCAAAACGCAATACTGTACATT110.308815272318922No Hit
TTGTTTGGTTTCATTGGAGAAGCAAGGAGTGGGCAAAACGCAATACTGTACATT110.308815272318922No Hit
ATTGTCTTCTATGGNGTCACAGGTAAGTGCTAAAATGGAGATCAAAACGCAATACTGTACATT100.2807411566535654No Hit
CTGCAGCGAGGAGTTNTGAGCAAAACGCAATACTGTACATT100.2807411566535654No Hit
ACAAACTGGTCAGTTGTGGGACCAAAACGCAATACTGTACATT100.2807411566535654No Hit
TGCTGGTGTAAAATTGGAATTGAAGCATGTGGAGGTGAAGGCAAAACGCAATACTGTACATT90.25266704098820886No Hit
TTGTTTGGTTTCATTTGAGAAGCAAGGAGTGGGCAAAACGCAATACTGTACATT90.25266704098820886No Hit
ATTGTCTTCTATGTTGTCACAGGTAAGTGCTAAAATGGAGATCAAAACGCAATACTGTACATT90.25266704098820886No Hit
CTGCAGCGAGGAGTTGTGGGCAAAACGCAATACTGTACATT90.25266704098820886No Hit
TTGGGATCCATAATATTGGTTTCATAATGCAAATGCCAAAACGCAATACTGTACATT90.25266704098820886No Hit
TTGGATCGGATGATNTGACAGAAGCAGGAGTCCTAGCCAAAACGCAATACTGTACATT90.25266704098820886No Hit
TTGTTTGGTTTCATNTGAGAAGCAAGGAGTGGGCAAAACGCAATACTGTACATT90.25266704098820886No Hit
ATTGTCTTCTATGGAGTCACAGGTAAGTGCTAAAATGGAGATCAAAACGCAATACTGTACATT90.25266704098820886No Hit
GGTTAACGTTGGANTGGAAAAGAGAAGCAAGAGGCAGTAAGGCAAAACGCAATACTGTACATT80.22459292532285235No Hit
ATTGTCTTCTATGTAGTCACAGGTAAGTGCTAAAATGGAGATCAAAACGCAATACTGTACATT80.22459292532285235No Hit
TTGGGATCCATAATNTTGGTTTCATAATGCAAATGCCAAAACGCAATACTGTACATT80.22459292532285235No Hit
GGTTAACGTTGGATNGGAAAAGAGAAGCAAGAGGCAGTAAGGCAAAACGCAATACTGTACATT80.22459292532285235No Hit
TTGGCATGAAGCATTGGAGTCCTAGCCAAAACGCAATACTGTACATT70.19651880965749577No Hit
CTTAGTAGTTGTCTAAGGAGATCAGGAACATGTAGCCCAAAACGCAATACTGTACATT70.19651880965749577No Hit
CTTAGTAGTTGTCTTAGGAGATCAGGAACATGTAGCCCAAAACGCAATACTGTACATT70.19651880965749577No Hit
AGAGGGATGTAACTAGAATTAACTGAGCTGAGCAAAACGCAATACTGTACATT70.19651880965749577No Hit
AGAGGGATGTAACNAAAATTAACTGAGCTGAGCAAAACGCAATACTGTACATT70.19651880965749577No Hit
GGGACGCGTTGGATTGCAGGGACAAAACGCAATACTGTACATT70.19651880965749577No Hit
CTGCAGCGAGGAGTNGTGAGCAAAACGCAATACTGTACATT70.19651880965749577No Hit
GGGGAGCGCCTCATTGAATTCGCCTACACCAAAACGCAATACTGTACATT60.16844469399213924No Hit
ATTGTCTTCTATGGTGTCACAGGTAAGTGCTAAAATGGAGATCAAAACGCAATACTGTACATT60.16844469399213924No Hit
ATTGTCTTCTATGNTGTCACAGGTAAGTGCTAAAATGGAGATCAAAACGCAATACTGTACATT60.16844469399213924No Hit
CTGCAGCGAGGAGTNGTGGGCAAAACGCAATACTGTACATT60.16844469399213924No Hit
TTGGATCGGATGATTTGACAGAAGCAGGAGTCCTAGCCAAAACGCAATACTGTACATT60.16844469399213924No Hit
GGTTAACGTTGGATTGGAGAAGAGAAGCAAGAGGCAGTAAGGCAAAACGCAATACTGTACATT60.16844469399213924No Hit
TTGGGATCCATAATTGTGGTTTCATAATGCAAATGCCAAAACGCAATACTGTACATT60.16844469399213924No Hit
ATGTCCCGGCGCTTGATGTGGCAAAACGCAATACTGTACATT60.16844469399213924No Hit
TTGTTTGGTTTCANTTGAGAAGCAAGGAGTGGGCAAAACGCAATACTGTACATT60.16844469399213924No Hit
GGGCTCTGCACCCTTGCGGCCCAAAACGCAATACTGTACATT60.16844469399213924No Hit
CTTGCACTGTGGANTCTCGGCAGGCCAAAACGCAATACTGTACATT60.16844469399213924No Hit
GGTTAACGTTGGATNGGAGAAGAGAAGCAAGAGGCAGTAAGGCAAAACGCAATACTGTACATT60.16844469399213924No Hit
TGTGTATGCAGTATTTGGAGTCCCGGAAGGTACAAAACGCAATACTGTACATT60.16844469399213924No Hit
GGGATTTGGCAGATTGGAGTCCTAGCCAAAACGCAATACTGTACATT50.1403705783267827No Hit
AGAGGGATGTAACNTAAATTAACTGAGCTGAGCAAAACGCAATACTGTACATT50.1403705783267827No Hit
CTGCAGCGAGGAGTTGTGATCAAAACGCAATACTGTACATT50.1403705783267827No Hit
ATTGTCTTCTATGTANTCACAGGTAAGTGCTAAAATGGAGATCAAAACGCAATACTGTACATT50.1403705783267827No Hit
GAAGGGCAGAAGATTGGAGTCCTAGCCAAAACGCAATACTGTACATT50.1403705783267827No Hit
GCCATGCCATGTATTGGTTATCTCTGAGTTTCTCCTCTTTTACTTTCATCAAAACGCAATACTGTACATT50.1403705783267827No Hit
GAAGTTCGGCGTCNACGAGTGCAAAACGCAATACTGTACATT50.1403705783267827No Hit
GATACGCAGAAGATTGGAGTCCTAGCCAAAACGCAATACTGTACATT50.1403705783267827No Hit
ATGTGGGATAGAATTGGAGTCCTAGCCAAAACGCAATACTGTACATT50.1403705783267827No Hit
TGTGTATGCAGTATCTGGAGTCCCGGAAGGTACAAAACGCAATACTGTACATT50.1403705783267827No Hit
ACAGGACCGGAGATNGGATCCTCCTATGTTGGAAATGAGGGAAGCAAAACGCAATACTGTACATT50.1403705783267827No Hit
ATGTCCCGGCGCTTTGTGTGGCAAAACGCAATACTGTACATT50.1403705783267827No Hit
TGTGTATGCAGTATTGGGAGTCCCGGAAGGTACAAAACGCAATACTGTACATT50.1403705783267827No Hit
GTTAGCCGGAATATTGGGGCCTCAAAACGCAATACTGTACATT50.1403705783267827No Hit
TTGGGATCCATAATAGTGGTTTCATAATGCAAATGCCAAAACGCAATACTGTACATT50.1403705783267827No Hit
GATAGAGAGCTTCTTGCACTGTAGAGTCCCAAAACGCAATACTGTACATT50.1403705783267827No Hit
TTGGATCGGATGANATGACAGAAGCAGGAGTCCTAGCCAAAACGCAATACTGTACATT50.1403705783267827No Hit
CTTAGTAGTTGTCTTGGGAGATCAGGAACATGTAGCCCAAAACGCAATACTGTACATT50.1403705783267827No Hit
AGAGGGATGTAACCNAAATTAACTGAGCTGAGCAAAACGCAATACTGTACATT50.1403705783267827No Hit
CTGCAGCGAGGAGNNGTGAGCAAAACGCAATACTGTACATT50.1403705783267827No Hit
TCGGATAGGAGCATTGGAGTCCTAGCCAAAACGCAATACTGTACATT50.1403705783267827No Hit
GGGACGCGTTGGACTCCAGGGACAAAACGCAATACTGTACATT50.1403705783267827No Hit
TGTGTGAGAAGCATTGGAGTCCTAGCCAAAACGCAATACTGTACATT50.1403705783267827No Hit
CTTAGTAGTTGTCTANGGAGATCAGGAACATGTAGCCCAAAACGCAATACTGTACATT50.1403705783267827No Hit
CTGCAGCGAGGAGTNGTGCGCAAAACGCAATACTGTACATT40.11229646266142618No Hit
GGGACGCGTTGGANTCCAGGGACAAAACGCAATACTGTACATT40.11229646266142618No Hit
AGGTCGGACGCGTTTGACTCCAGGGACAAAACGCAATACTGTACATT40.11229646266142618No Hit
GGTTAACGTTGGANNGGAAAAGAGAAGCAAGAGGCAGTAAGGCAAAACGCAATACTGTACATT40.11229646266142618No Hit
GGCACATGGAGCATTGGAGTCCTAGCCAAAACGCAATACTGTACATT40.11229646266142618No Hit
GTACGAGGCGATATTGAGTCCTGCCAAAACGCAATACTGTACATT40.11229646266142618No Hit
ACAAACTGGTCAGATGTGAGACCAAAACGCAATACTGTACATT40.11229646266142618No Hit
GAAGTTCGGCGTCAACGAGTGCAAAACGCAATACTGTACATT40.11229646266142618No Hit
TTGGGATCCATAANATTGGTTTCATAATGCAAATGCCAAAACGCAATACTGTACATT40.11229646266142618No Hit
TTGTTTGGTTTCATCNGAGAAGCAAGGAGTGGGCAAAACGCAATACTGTACATT40.11229646266142618No Hit
GATTTATGGTGGATTGGAGAGGTTTCTAACAGATCGATATCTCCAAGGGCAAAACGCAATACTGTACATT40.11229646266142618No Hit
CTGCAGCGAGGAGATGTGGGCAAAACGCAATACTGTACATT40.11229646266142618No Hit
CTGCAGCGAGGAGGNGTGAGCAAAACGCAATACTGTACATT40.11229646266142618No Hit
CTTAGTAGTTGTCNTAGGAGATCAGGAACATGTAGCCCAAAACGCAATACTGTACATT40.11229646266142618No Hit
AGGGGGAGGGACATTGGATGGACCCAAAACGCAATACTGTACATT40.11229646266142618No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN40.11229646266142618No Hit
AGGTCGGACGCGTTGNACTCCAGGGACAAAACGCAATACTGTACATT40.11229646266142618No Hit
ATTGTCTTCTATGNAGTCACAGGTAAGTGCTAAAATGGAGATCAAAACGCAATACTGTACATT40.11229646266142618No Hit
CTGCAGCGAGGAGTGGTGAGCAAAACGCAATACTGTACATT40.11229646266142618No Hit
AGAGGGATGTAACTTGAAGTAACTGAGCTGAGCAAAACGCAATACTGTACATT40.11229646266142618No Hit
TTATGGGTAATGATTAGAGTCCCAAAACGCAATACTGTACATT40.11229646266142618No Hit
AGAGGGATGTAACTAAAATTAACTGAGCTGAGCAAAACGCAATACTGTACATT40.11229646266142618No Hit
CTTGCACTGTGGATTGTCGGCAGGCCAAAACGCAATACTGTACATT40.11229646266142618No Hit
TTCACCCTTTGATTTGTTCTTTCTTTTTGTCTCACTGTGTCAAAACGCAATACTGTACATT40.11229646266142618No Hit
CTGCAGCGAGGAGTTNNGNGCAAAACGCAATACTGTACATT40.11229646266142618No Hit
CTTGCACTGTGGATTCTCGGCAGGCCAAAACGCAATACTGTACATT40.11229646266142618No Hit
AGAGGGATGTAACTANAATTAACTGAGCTGAGCAAAACGCAATACTGTACATT40.11229646266142618No Hit
AGAGGGATGTAACTTAAATTAACTGAGCTGAGCAAAACGCAATACTGTACATT40.11229646266142618No Hit
CTTAGTAGTTGTCTNAGGAGATCAGGAACATGTAGCCCAAAACGCAATACTGTACATT40.11229646266142618No Hit
ACAGGACCGGAGANTGGATCCTCCTATGTTGGAAATGAGGGAAGCAAAACGCAATACTGTACATT40.11229646266142618No Hit
CTGCAGCGAGGAGTTGGGAGCAAAACGCAATACTGTACATT40.11229646266142618No Hit
TTGTTTGGTTTCANCTGAGAAGCAAGGAGTGGGCAAAACGCAATACTGTACATT40.11229646266142618No Hit
CTGTGGAGAGAGATTGGAGTCCTAGCCAAAACGCAATACTGTACATT40.11229646266142618No Hit
ATTGTCTTCTATGTGGTCACAGGTAAGTGCTAAAATGGAGATCAAAACGCAATACTGTACATT40.11229646266142618No Hit
ACTAGTGACAGAATTGGAGTCCTAGCCAAAACGCAATACTGTACATT40.11229646266142618No Hit
AGAGGGATGTAACTTGAATTAACTGAGCTGAGCAAAACGCAATACTGTACATT40.11229646266142618No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)