FastQCFastQC Report
Wed 28 Jun 2023
EGAF00008082268

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008082268
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences463794
Sequences flagged as poor quality0
Sequence length35-151
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN127812.755749319741092No Hit
TAGCTCCCCTTCCCGATGTTCTCAAACAACTTGTAGATGTTGTCCCCTTC110592.3844637921146026No Hit
GTCTCACCACTGACCAGTTTGTCTGTCTGTGTGTGTCCATGTGCGAGGGC83821.8072678818613435No Hit
GCACCGTGAAGTCCTGAGTGTAGATGATGTCATCCTCGATGTCGAAGAGG77931.6802718448276606No Hit
AGGCCCCAGTATTCCGGCTAACCACTCCCAGCCCCTGGGCCGGCCCCCTA71881.5498260003363562No Hit
CTCCCCGGTGCGCATGTACTGCGCGGCTGGCTGGCTGTTGCAGCATGTGC67551.4564655860144806No Hit
TCCCTGGAGTCCAACGCGTCCATGAGCTCCAACACACCACTGGTGCGCAT59351.2796629538113904No Hit
CTGCTGTGGGAGATCGTGACCCTAGGGGGAAACCCCTATCCTGGGATTCC58711.2658637239809054No Hit
GCTCGAGCTCGGAGACATTGGCCAGCGTGGGGCCCTCCCCTGAGGACAGC58591.2632763683876895No Hit
GCAGTTGGCGGGCTTGTCCATGCGGTGGCCCTCCTTCAGCAGCTTGAAGA57831.2468897829639882No Hit
CGCCCAGCAGGTTGATGATGTTTTTGTGTTTCCCGATCATCTTCATCATC54841.1824215060996908No Hit
GCATGAGCACATTGTCAAGTTCTATGGAGTGTGCGGCGATGGGGACCCCC52891.1403769777099315No Hit
GTGGAGCCTGGTCATGGAAAGCGTGGTGCCCTCGGACCGCGGCAACTACA51081.101351030845591No Hit
TCGGCGCCTTTCGAGCAGTACTCCCCGGGTGGCCAGGACACCCCCAGCTC49991.077849217540546No Hit
TTGGTTTGAAAGACAAGCAGGGATGTCTATAGGGAAGGGAAGACGGGGCC49921.0763399267778366No Hit
GCCATCAGCACTGGAGTCTCGTGATGGAGAGCGTGGTGCCCTCGGACCGC49461.0664217303371755No Hit
TGTGTGTACACCCGGTCAAACAAGGCCTCGGGCGCCATCCACTTCACAGG49411.0653436655066688No Hit
TCAGGCTCCTGGGAGTTCTATCCTCCCAGCCTATCCCCTCTCCTTTTCTT47711.028689461269443No Hit
CTGTGCCAGGGACCTTACCTTATACACCGTGCCGAACGCACCGGAGCCCA46541.0034627442355872No Hit
GGCCGTTAGGGTGCAGAGCCAAAGCTTTGGCAGCCTCTCCACGCTCCCTC45680.9849200291508731No Hit
GGTACCAGGAGATGATGTAAGGGACAAGCAGCCACACCCCATTCTTGAGG45180.9741393808458065No Hit
ATCTCACAATTGCCAGTTAACGTCTTCCTTCTCTCTCTGTCATAGGGACT44930.9687490566932734No Hit
CCTTCTGGAAAAGTAGCTCGGTAGTCTACAGATTCATTTGAAACCATTTC44690.9635743455068414No Hit
GTGGGCTTTCTCCTCTGTCTCTCCGGTGGCCCCAGGCAATCGACTGCATC44240.9538717620322816No Hit
GTGGAGCCTGGTCATGGAAAGCGACCCGTACGTGCTGATCGAAGGGATGA43550.9389944673712899No Hit
TGGAGGTGGTGTCCATTGAGGGTATCCACCCCAAGGTCATGGAGCGCCTC43380.9353290469475672No Hit
GCGACTGCCCTCCTTGTACCAGTGGCCACCACGCTCAGCCCGCCCACAGC43000.9271357542357167No Hit
GGTCCATCCGATGTCCCTCCCGCAGCAGCGAGAACAGCTCCTCCACCGGG42230.9105335558459142No Hit
CCCAAAGGGCAGTAAGATAGGAAACAGTGTCTCACGCATACGGTTTGGTT41440.8935001315239093No Hit
CCACATCAAGCGCCGGGACATCGTCGCCCCCCTGCTTCAGCAACGCCTTC40600.8753886423713976No Hit
TGCCAAGACAGTCCCCGCTACAACCCCAGCCCTCCCAAGACTGGGGCTAC40160.8659016718629391No Hit
GGTAGATGACCTCGGTGGAGTCGATGCGGTGGATGAACGTGTCCATACCC38580.831834823218929No Hit
TGAACGGTGCTGTCATGTACCAGATCGACAGCGTTGTCCGTGCCTGCAGT38120.8219166267782679No Hit
GCCCCACTTTGCGGCTCTCGCGGAGGAAGCCCCGCAGGGAGCCGTATTTG37350.8053144283884656No Hit
AGGCTGAGTGGGCTACGTCTGCCCTCAGGGGGCAGCATTGTTGGGGGACA36310.7828906799139272No Hit
CATCTTGGGCCTGTGTTATCTCCTAGGTTGGCTCTGACTGTACCACCATC36150.7794408724563061No Hit
GCCTGGCACACTGGGAAACGCTTCTCCGGCACGCTGTCCAGCATTTCCTG35550.7665040944902263No Hit
GCCCCACTTTGCGGCTCTCGGCGCTGATGCTCACGTGCGGCACCTACGAC35290.7608981573715917No Hit
GCAGCTCAGAACCTGGTATCTACTTTCTGTTACCTGTCGCTTGAGCGGGA34630.7466677016089039No Hit
CCCTGGACCACCCGGATGAGCGGGCCCTCACCATGGGCGACCTCATCTCA34570.745374023812296No Hit
CCATTTTGGGTCCTCTGGAAGTTCATACTCGGAGACCCCTGCCAGCATGG34430.7423554422868773No Hit
GGCCCACCTTGAGCACGGTAACGTAGGGTGTGCCGTCCGGGCCCACCTTG33370.7195004678801364No Hit
GCTCCTCTTCACGTAGGAATCCTCTTCATAAACATCTCGGGACAAGCCGA33170.7151882085581098No Hit
GTGATCAAGAAATCGATAGCATTTGCAGTATAGAGCGTGCAGATAATGAC32610.7031138824564354No Hit
GCCGAAGAAGCGCACGATGTGGCCGAGGAACTGCGTGACCACACGCGAGC32550.7018202046598274No Hit
CGTGCAGGTCCTCCAAGTACGGCACCACAGTCATGCTGCGCCACCGGTCC30850.6651660004226014No Hit
GCCGCCACCACCAGGATGAACAGGAAGAAGCCCACCCCGTAGCTGAGGAT30340.6541697391514336No Hit
CGGCACTCGGCTCCTTTCTGTAGCTGGCGTGGCCCCAGAGCTCACCTGTC29090.6272181183887674No Hit
CACTCGTTGACGCCGAACTTCCTGGCGGAAGTTGTCGAGCTGGTCGACGG28480.6140657274565863No Hit
CGCAATGTGCTGGTGACCGAGGCTGGCGGCCTGGTGGGCAATTTCACGGG28200.608028564405749No Hit
CCGTCGGGCGAGTTGTTCCTGCCGCCCACGGCGTACAACAGCCCGCCCAC28090.6056568217786344No Hit
GTCCTTAGCGCCCCACGTATATGGTGATGGAGTACTGCGTGTGTGGCATG28040.6045787569481278No Hit
AAAGTAGAAAATGGAAGTCTATGTGATCAAGAAATCGATAGCATTTGCAG27810.5996196587277972No Hit
GCAGTTGGCGGGCTTGTCCATCACGACCTGTAATCGCCGTTGATCGCGAC26970.5815081695752856No Hit
TCCCTGGAGTCCAACGCGTCCCTCGGCGAGATGATCAGCCAGCTGGCCCA26540.5722368120329284No Hit
CGCAGGTACTTCTGTCAGCTGATTGACGGCCTGGAGTACCTGCATAGCCA25440.5485193857617822No Hit
TCCCTGGAGTCCAACGCGTCCTGCAAATCGGCATGCAGCGAACCGTTGCC25100.541188544914337No Hit
GGGTGAAGAGGAATCCCAAAGTTCCAAACAAAAGAAATGCAGGGGGATAC24700.5325640262702838No Hit
GGCCTCTGATTCCTCACTGATTGCTCTTAGGTCTGGCCCCTCCTCAGCAT23820.5135900852533668No Hit
CCCGTCTGAGGAGTACGTTGGGAAGGATCGGTCTGGGGAAAAGGGCCCTA23740.5118651815245562No Hit
TACGCATTCATGAACACATACTTATTCTAACTTCTGACTTTTAACCACAT23650.5099246648296442No Hit
CCCATGAGCGTGCCCCGTAACCGCATCGGGGTGGGGGTCATCGATGGCCA23630.5094934388974416No Hit
GCGGGCTGTTGTACGCCGTGGCGATCGTCGGTGCGCAGATCGTGTTCCAG21950.47327046059241823No Hit
GGTGTCTCTCTGTGGCTTTACCTGATGATCAGGGCTTCCATGAGGAAATC21800.4700362661008982No Hit
TCCCTGGAGTCCAACGCGTCCGCGGCGGCCTGCGCGTATTCGGGCGCGTC21300.45925561779583174No Hit
GGGTTAATGGACAATGAAAAAGACATGTAAGCAAGGCGCTAGCTCTGTGG20830.4491218083890693No Hit
CCAGAGGCTAGCAGTTCAACTTCTGTAACACCAGATGTTAGTGACAATGAAAGGACTCCAATCTAAACGAGAA20650.44524077499924536No Hit
GTGTAGGCGAATTCAATGAGGCGCTCCCAATGTAGGCAAAAAGCCGCCCC20630.44480954906704273No Hit
GCCGAAGAAGCGCACGATGTGCTGCGGCGCAAGCGTCAGTACGTCGACCG19850.427991737711139No Hit
CAACGCCACCACAAGATGATAAGTCACAGGCTGGAAGACTAGGGGGGCTC19710.42497315618572035No Hit
CCAGAGGCTAGCAGTTCAACTTCTGTAACACCAGATGTTAGTGACAATGA19320.4165642505077685No Hit
CCACATCAAGCGCCGGGACATCGCGGGGGGAAATTCCCTAAGACCGCTGC18940.40837095779591803No Hit
GTGTAGGCGAATTCAATGAGGCGCTCGCGTATTGCGCACGCGTGACGGCG18750.40427431143999276No Hit
CCAGGTGGTCTTTTGGGATCCTGCTATGGGAGATCTTCACCCTCGGGGGC18120.39069069457560895No Hit
GATGCCACTGACAAGGACCTGTCGGACCTGGTGTCTGAGATGGAGATGAT18060.389397016779001No Hit
TTGAGGTCCCCATGCTTCATGTACTCAAAGACCATGATGAGGGGGTCGCC17940.3868096611857851No Hit
CGCAATGTGCTGGTGACCGAGGCGGGTGGCGTGGTGACCGCGCTGGATGG17800.38379107966036646No Hit
GCAGTTGGCGGGCTTGTCCATCACGCCCGGGATGTTCGTCATCATGACGA17420.3755977869485159No Hit
CCTCACTCTAACAAGCAGATAACTTTCACTTAATAGTTGTTTTAGAAGAT17270.372363592456996No Hit
GCCTGCCGAGATTCCACAGTGCGCGCGGATCGAACAGCACCGACGCCATC16850.3633078478807401No Hit
AGGCCATCATCCTGACGTTGGGTCGGCTGATACACCCCTGTCCTCTCTGT16420.35403649033838297No Hit
GCAGTTGGCGGGCTTGTCCATGCGGTGGCCCTCCTTCAGCAGCTTGAAGGACTCCAATGCTGGCCCTAGG15910.3430402290672152No Hit
TTTCTGTCATCCAAATACTCCACACGCAAATTTCCTTCCACTCGGATAAG15890.3426090031350125No Hit
GAAGAAAACGGCATTTTGAGTGTTAGACTGGAAACTTTCCACTTGATAAG15890.3426090031350125No Hit
GCTCGAGCTCGGAGACATTGGCTGGCAGGGCAGTGTACGGAGAGTGCCTT15510.33441571042316204No Hit
AAACCTGTCTCTTGGATATTCTCGACACAGCGGCAAACCCGCTCGAATAG14580.31436370457573837No Hit
GGTCCAGATGAAGCTCCCAGAATGCCAGAGGCTGCTCCCCGCGTGGCCCC14430.31112951008421846No Hit
ATGTCACCACATTACATACTTACCATGCCACTTTCCCTTGTAGACTGTTC14280.3078953155926985No Hit
CCACATCAAGCGCCGGGACATCGCGGAAAACGCACTCTGGCTTTGATAGC14180.3057391859316852No Hit
GTCATCTTCTGTCCCTTCCCAGAAAACCTACCAGGGCAGCTACGGTTTCC14080.3035830562706719No Hit
GCAGGACGATCATGGAGCCTCGTGCCAGGCAGAGGTAGCGGCCAGCATCCTCAGGACTCCAATATCACCTCTAGC13960.30099570067745596No Hit
CCATGGCTGCTTTCCATAGGTGAAGATCTCCCAGAGGATCACCCCGAAGC13870.299055183982544No Hit
GCCCTCCCCCAATCAAGATTCCTACGCACCCCCTTTTTACCTCCGTGTTT13820.29797711915203734No Hit
GCAGTTGGCGGGCTTGTCCATCGATGGCCTGCTCGCCGCGGGGGACTTCA13800.29754589321983466No Hit
CCCACGCTCTTCTCACTCATATCCTCCTCTTTCTGCCCAGGGCATCTGGA13630.29388047279611207No Hit
GCGGGCTGTTGTACGCCGTGGACCGCAAGCTGGTCGGCCCGTCGTTCGCC13600.29323363389780804No Hit
GGTCCATCCGATGTCCCTCCCGCCTTGTATTCGTTGAACGCGATCTCGGC13490.29086189127069345No Hit
CCAGGTGGTCTTTTGGGATCCTGCTATGGGAGATCTTCACCCTCGGGGGAGGACTCCAATGCTTGCCTCCTC13360.2880589227113762No Hit
GTCTCACCACTGACCAGTTTGTCTGTCTGTGTGTGTCCATGCGCGAGGGC13250.2856871800842615No Hit
GCGGGCTGTTGTACGCCGTGCCCGGTCGTGCCAGGACTCCAATCAAAAGAGACCT12900.278140726270715No Hit
TCCCTGGAGTCCAACGCGTCCACGAAGCGCGCCGTCTCGAGCGAGATGCC12580.27124111135547246No Hit
CCCCCAACCCTACATTTCTGCACAAAAGCCCCGCCTCCCTGGGGCAGGACTCCAATGAGATAGCTAGC11490.24773929805042755No Hit
CTCCACTTCCAACTCCTAAGATGTCCACTGTCTCTTATAACATCTCCGAGCCAGGACTCCAATCATCCGGGAGTC11370.2451519424572116No Hit
GCCGAAGAAGCGCACGATGTGGCTTTCACGCCGGTGCGGTTCGTCAAGTC11270.24299581279619833No Hit
AGGGATGAGCTTGACTTGCCGGAAGAGCCTGACTCCAGGGAGGACTCCAATTGAACATACACT11260.242780199830097No Hit
CAGCCGCCCGAGATTGCCAACCCGGATCACGCGTGCACGCGTCGCGAGGG11260.242780199830097No Hit
AGGCCCCAGTATTCCGGCTAACCAGCTTTCCATCGGGCTCAGCGTGAGGC11050.23825232754196907No Hit
CGCAATGTGCTGGTGACCGAGGCCACACGCTCGACGCGGCGCCGCTCGCA11000.2371742627114624No Hit
GTGTAGGCGAATTCAATGAGGCGCTCAAGGGACGGCGCGACCGCGTGCTG10600.22854974406740924No Hit
CCACATCAAGCGCCGGGACATCAGCAGATCCACCTCGTCCTCCGAATAGG10380.22380625881318No Hit
CGCAATGTGCTGGTGACCGAGGGCGCGCTGGTCGGCCAGGGCGAAGCCAC10380.22380625881318No Hit
TACCTTCCGGGACTCCAGATACTGCATCGGAGCTGTCTAGGACTCCAATGTGTTGCCCCTG10310.2222969680504707No Hit
GCCTGCCGAGATTCCACAGTGCTGGGATTACAGGTGTGAGCCACCGCCCC9990.21539735313522818No Hit
GCCTGGCACACTGGGAAACGCAGCGCCGTGCCGAGATCGCTCGCGACCGG9320.20095128440643906No Hit
GATTCTGTCAGCATTACTCTGTGTCCCGTTAAAAGGACTCCAATGCCCCTTTATGG9230.1990107677115271No Hit
CCCTGGACCACCCGGATGAGCCGCCCGACGACCCGTTATTGGTGATTCCC8850.19081747499967658No Hit
GGTTTTCCCGGACATGGTCTAAGAGGCAGCCATAGGGCAAGGACTCCAATAGAAGTAGATAG8640.18628960271154868No Hit
GGCCGTTAGGGTGCAGAGCCTCGATCGTAAAAGGCAGAAGTTCAGTGGAG8610.18564276381324468No Hit
GGCCGTTAGGGTGCAGAGCCAGGGGGCCCTGGGGTGCAGAGTCAAGGGGC8300.17895876186410348No Hit
GTGGAGCCTGGTCATGGAAAGCGGCCTAGACGGCACGGAGAACTGCAGTA8200.17680263220309017No Hit
CTGTGCCAGGGACCTTACCTTATACACCTCTCCGAGCCCACGAGACAGGACTCCAATCTGCGTGTAGCC8160.17594018033868483No Hit
CCACATCAAGCGCCGGGACATCCCAAGGAACTCAATACCGAGCCGTTTTT8140.17550895440648218No Hit
GAGGCCAGTCACAATGGACAGCGCAAATCCCGAGAGCGGGCTCTCGCATT8110.1748621155081782No Hit
GCAGGACGATCATGGAGCCTCGTGGCCGCGACCAAGCGCCTCAAATTCCT8070.1739996636437729No Hit
TCTGCGAGCCCGAAGACATCCGCGCACGCGGCATGACGCTGTCCACCGGC8040.1733528247454689No Hit
CTCTGACTGCTCTTTTCACCCATCTACAGTCCCCCTTGCCGTCCCAAGCA7960.17162792101665825No Hit
GCATTTGCATTATGAAACCAATATTATGGATCCCAA7660.16515953203361836No Hit
AGGTGAAGCCAAACCTGCTCACCCGCCAATGCGTACAGCGACATTGGTGC7570.16321901533870642No Hit
GCAGGACGATCATGGAGCCTCGTGTCCTGACACCGAAGCCCTAACGCGGG7560.16300340237260508No Hit
TGTGTGTACACCCGGTCAAACAAGGCCTCGAGCGCCATCCACTTCACAGG7270.15675062635566653No Hit
CCACATCAAGCGCCGGGACATCACGGCAGTGTTCGAGAAAAAGGAGTGAG7190.1550257226268559No Hit
TCGTTTTTGACAGTTTGACAGTTAAAGGCATTTCCTGTGAAATAATACTG6990.15071346330482932No Hit
CCCCCAACCCTACATTTCTGCACAAAAGCCCCGCCTCCCTGGGGCTGCGG6920.14920417254211998No Hit
GCAAGCAAGGGTTCAAAGACCCAAAACCCAAAATGGCAGGGGAAGGACTCCAATAGGATTTGTACG6900.14877294660991733No Hit
ATGTCGGTGGGTATGGACACGTTCATCGCGCAGGCGACCAACACGCCGCT6900.14877294660991733No Hit
GCAGTTGGCGGGCTTGTCCATGCGGCCCAAAAACATGCGTGCGTAGCTGG6770.14596997805060005No Hit
GCAGCAGGTCGTGGGCAAACATCGCCGCGCGCATGCCCGAGCCGCACATC6600.14230455762687744No Hit
CCACATCAAGCGCCGGGACATCCACGTCCACGTTCCGGAGGGGGCGACCC6550.14122649279637078No Hit
CCGAGGCCAGGTATACGGACATCATCCTGTACGCGTCGGGCTCCCTGAGGACTCCAATGCAGTCACCTCC6280.1354049427116349No Hit
GTGTAGGCGAATTCAATGAGGCGCTCGCCATCCAGCAGCGCTGCGTTGTC6230.13432687788112824No Hit
CACTCGTTGACGCCGAACTTCCTGACGATGTTGAAACGCTGCGAGCCTTG6180.1332488130506216No Hit
CGCAATGTGCTGGTGACCGAGGCCGTGGGCCATCTGTCGGCCCACGAGGT6170.1330332000845203No Hit
GGTAATCTACTGGGACGGAACAGCTTTGAGTTGATGACGTCCCATGCACG5980.12893655372859503No Hit
CTCAGCTCAGTTAATTTTGGTTACATCCCTCTCTGCAGGACTCCAATCCGATCTGCGCC5960.12850532779639234No Hit
GCCATCAGCACTGGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA5900.1272116499997844Illumina PCR Primer Index 3 (96% over 27bp)
CCGTGTTGGAGAGCTGGTACCAGGCGCGGCTGGAGGGCGGCCGCCAGTTC5790.12483990737266976No Hit
CCCATGAGCGTGCCCCGTAACCGCTCGGGCGTCGCCACGCCGCACAATCG5600.1207432610167445No Hit
CCTTCACCTCCACATGCTTCAATTCCAATTTTTAT5440.11729345355912323No Hit
CGCAATGTGCTGGTGACCGAGGTGGGCATCTCGACGATGCACGGGTCCGA5350.11535293686421126No Hit
CCACATCAAGCGCCGGGACATCTGCCCGAGCCCGAAGCCAACGGCGTGCG5250.11319680720319797No Hit
CAGCCGCCCGAGATTGCCAACCACTACACGGCGCTTGCTCATTTGTTCAC5200.11211874237269132No Hit
GTGTAGGCGAATTCAATGAGGCGCTCGCCGACGGGCGTTTGCAGCAAGTG5080.10953138677947537No Hit
CGCAATGTGCTGGTGACCGAGGCCCTGGCCCGCTGGCACGTCCTCGGGAT4990.10759087008456342No Hit
GCCTGGCACACTGGGAAACGCGGTTTCATGTGCGCGCACATGGCGAACAA4860.10478790152524613No Hit
GCCTGGCACACTGGGAAACGCCTATTTTCGGCCGCAGCGTGCGGCCCTCG4820.10392544966084082No Hit
TGCTCTGGGAGATCTTCACGCTGGGGCAGCAACTCCCACGGGACTGGCGT4720.10176931999982752No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)