FastQCFastQC Report
Wed 28 Jun 2023
EGAF00008086162

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008086162
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10322373
Sequences flagged as poor quality0
Sequence length35-76
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN4507624.366844716810757No Hit
GGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGTAA1274951.235132657965373No Hit
GCCAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCGGCC637630.617716488253234No Hit
GGTCTAGCGCCAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGA436680.42304225975945653No Hit
GGTTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGTAA435910.42229630725415557No Hit
CGTTGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG393950.3816467395626955No Hit
CTGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGT376980.3652067213614544No Hit
GGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCGGCCCCCTTTCCGGCCGCACCCCGTTTCCCA364980.35358148751260976No Hit
GGCTTTCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGTAA333560.32314275021838484No Hit
TCTGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTG327660.3174270102427029No Hit
TGGTCTAGCGCCAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAG325040.3148888341857052No Hit
TGCCAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCGGC300810.29141554950591303No Hit
TGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGTA289850.2807978359239683No Hit
GGTTTAGCGCCAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGA278160.2694729206162188No Hit
GCGCCAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCGG275930.26731256465930847No Hit
GCGTTCAACGTGACGGGCGAGAGGGCGGCCCCCTTTCCGGCCGCACCCCGTTTCCCAAGACGAACGGCTCTCCGC275580.26697349533871717No Hit
CCAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCGGCCCCCTTTCCGGCCGCACCCCGTTTCCC268640.2602502350961354No Hit
CGCCAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCGGC263320.25509638142314756No Hit
TGTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG233030.2257523536496889No Hit
GACGGGTCGGGTGGGTAGCCGACATCGCCGCCGACCCCGTGCGCTCGGCT206130.19969245443852884No Hit
ACGTGCGTTCAACGTGACGGGCGAGAGGGCGGCCCCCTTTCCGGCCGCAC199570.19333732660116043No Hit
AGCCAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCGGC198050.19186479698030676No Hit
AGCCTACAGCACCCGGTATTCCCAGGCGGTCTCCCATCCAAGTACTAACCAGGCCCGACCCTGCTTAGCTTCCG190970.18500590900948843No Hit
TGGTTTAGCGCCAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAG190540.18458933812990483No Hit
TCCGCTGACTAGTTTCTTTTCCTCCGCTGACTAGTTTCTTTTCCTCCGCT172850.167451805897733No Hit
TAGCGCCAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGC165900.16071885796027716No Hit
GCCTACAGCACCCGGTATTCCCAGGCGGTCTCCCATCCAAGTACTAACCAGGCCCGACCCTGCTTAGCTTCCG156140.15126366776321684No Hit
TGTTGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG153470.1486770532318489No Hit
AGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCGGCCCCCTTTCCGGCCGCACCCCGTTTCCCA145340.14080095729925668No Hit
CGGCTTCGTCGGGAGACGCGTGACCGACGGTCCCCCCGGGACCCGACGGC144620.140103443268326No Hit
CCGCTGACTAGTTTCTTTTCCTCCGCTGACTAGTTTCTTTTCCTCCGCTGACTAGTTTCTTTTCCTCCGCTGACT135360.13113263781496753No Hit
GGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGG133260.12909822189141973No Hit
AGTGCCAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCG132360.12822632935275638No Hit
AGCGCTAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCG131070.1269766167140056No Hit
TTTGGGTCCTAACGCGTACGCTCGTGCTCCACCTCCCCGGCGCGGCGGGC130830.12674411203702868No Hit
GGGGACAGTCCGCCCCGCCCCCCACCGGGCCCCGAGAGAGGCGACGGAGG128660.12464188224936262No Hit
TGCGCCAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCG123500.11964303169435943No Hit
GCGCTAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCGG123360.11950740396612292No Hit
GTGCCAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCGG123210.11936208854301233No Hit
ATGGTTTAGCGCCAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGA122170.11835456827611249No Hit
CAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCGGCCCCCTTTCCGGCCGCACCCCGTTTCCCA121380.11758924038106353No Hit
GTTCAACGTGACGGGCGAGAGGGCGGCCCCCTTTCCGGCCGCACCCCGTT115960.11233850975933538No Hit
CGTGCGTTCAACGTGACGGGCGAGAGGGCGGCCCCCTTTCCGGCCGCACC115830.1122125697259729No Hit
ATGGTCTAGCGCCAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGA115760.11214475586185464No Hit
GGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGTA115650.11203819121824023No Hit
GGGTCAGTGAAAAAACGATGAGAGTAGTGGTATTTCACCGGCGGCCCGCG114780.11119536176419897No Hit
CGTCCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGGC114410.11083691705385962No Hit
GGCTACCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGTAA113000.10947095207662037No Hit
CAACGTGACGGGCGAGAGGGCGGCCCCCTTTCCGGCCGCACCCCGTTTCCCAAGACGAACGGCTCTCCGCACCGG112620.10910281967140695No Hit
ACGGGTCGGGTGGGTAGCCGACATCGCCGCCGACCCCGTGCGCTCGGCTT112320.10881218882518584No Hit
TCAACGTGACGGGCGAGAGGGCGGCCCCCTTTCCGGCCGCACCCCGTTTC112320.10881218882518584No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)