FastQCFastQC Report
Wed 28 Jun 2023
EGAF00008086163

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008086163
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10322373
Sequences flagged as poor quality0
Sequence length35-76
%GC68

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN4061403.9345603961414684No Hit
GGGAGCCGGGTCCCCGTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCC1145451.109677009346591No Hit
CGGAGCCGGGTCCCCGTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCC985850.9550613991569574No Hit
GGCAGCCGGGTCCCCGTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCC855710.8289857380662372No Hit
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG730920.7080929937331271No Hit
GGGGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTC686940.6654865116771115No Hit
CGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG380600.3687136669058559No Hit
CGGGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTC364900.3535039859536175No Hit
GGCCCGGCGGATCTTTCCCGCTCCCCGTTCCTCCCGACCCCTCCACCCGC358470.34727479814961154No Hit
CGCAGCCGGGTCCCCGTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCC304100.29460280111947124No Hit
GGGCGCCGGGTCCCCGTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCC270550.26210058481707643No Hit
GGGGCCGGGCGCGGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGACAGGAGGATCGCTTGAGTCC269190.2607830583142074No Hit
GCCAGCCGGGTCCCCGTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCC264590.2563267186721503No Hit
GCGAGCCGGGTCCCCGTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCC253670.24574775586970166No Hit
GCGGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTC248980.24120422697377822No Hit
CCGAGCCGGGTCCCCGTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCC243860.23624412719827118No Hit
CGGGAGCCGGGTCCCCGTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTC240340.23283405860261008No Hit
GGGGGGTCGGTCGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGG230840.22363074847227474No Hit
CGGCGCCGGGTCCCCGTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCC226920.21983317208165215No Hit
GCGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG210060.20349971852402543No Hit
GCCCCGGCGGATCTTTCCCGCTCCCCGTTCCTCCCGACCCCTCCACCCGC209440.20289908144183513No Hit
CCGGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTC204060.19768710159960312No Hit
GGCCGCCGGGTCCCCGTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCC201900.1955945595068111No Hit
GGCGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG201690.1953911179144563No Hit
CGGACGGCAGCGCCGAAGGAGCCTCGGTTGGCCCCGGATAGCCGGGTCCC199810.19356983127813732No Hit
CGCCCGGCGGATCTTTCCCGCTCCCCGTTCCTCCCGACCCCTCCACCCGC199360.19313388500880563No Hit
CGGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTC190460.18451183657091252No Hit
GGGGAAGGGCCCCGCCCGGGGGCCCGAGGTGGGATCCCGAGGCCTCTCCA185790.17998768306473717No Hit
CCGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG185090.17930954442355454No Hit
CGGCCGGCGGATCTTTCCCGCTCCCCGTTCCTCCCGACCCCTCCACCCGC182850.1771395007717702No Hit
GGGTGCCGGGTCCCCGTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCC175450.16997060656498267No Hit
GGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGTAA170390.16506863295871987No Hit
CGGCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCCCGCGGCGGCGCGGG164730.1595853976600148No Hit
CGGGCCGGGCGCGGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGACAGGAGGATCGCTTGAGTCC158470.15352090066886753No Hit
GGGCCGGCGGATCTTTCCCGCTCCCCGTTCCTCCCGACCCCTCCACCCGC156240.15136054471195723No Hit
GGGACGGCAGCGCCGAAGGAGCCTCGGTTGGCCCCGGATAGCCGGGTCCC156220.15134116932220915No Hit
GGCTGCCGGGTCCCCGTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCC141930.13749745334720997No Hit
CGGTGCCGGGTCCCCGTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCC141550.13712932094199656No Hit
CGGGGGTCGGTCGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGG139410.13505615423895262No Hit
GGGCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCCCGCGGCGGCGCGGG130420.12634691654719316No Hit
GGCACGGCAGCGCCGAAGGAGCCTCGGTTGGCCCCGGATAGCCGGGTCCC123910.12004022718419495No Hit
CGGGACGGCAGCGCCGAAGGAGCCTCGGTTGGCCCCGGATAGCCGGGTCC123410.1195558424404931No Hit
GGGGGCAGGGGAGATACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGG119950.11620390001407623No Hit
GGGAGTCAGCGGAGGAAAAGAAACTAGTCAGCGGAGGAAAAGAAACTAGT119600.11586483069348492No Hit
GCCGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG117970.11428573642901685No Hit
GGCAGTCAGCGGAGGAAAAGAAACTAGTCAGCGGAGGAAAAGAAACTAGT113430.10988752295620398No Hit
GGCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCCCGCGGCGGCGCGGG110300.10685527446063033No Hit
GCGGCCGGGCGCGGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGACAGGAGGATCGCTTGAGTCC108750.10535368175515457No Hit
CCGGCCGGGCGCGGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGACAGGAGGATCGCTTGAGTCC106770.10343551817009519No Hit
GGGGGCCCCGGATAGCCGGGTCCCCGTCCGTCCCCGCTCGGCGGGGTCCC104420.10115890987469645No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)