FastQCFastQC Report
Wed 28 Jun 2023
EGAF00008086172

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008086172
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13787622
Sequences flagged as poor quality0
Sequence length35-76
%GC68

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGG11465508.31579223741411No Hit
GTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGGC3351002.4304408693536854No Hit
GGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGGCGG3217472.333593131578455No Hit
TGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGG2598421.8846034508343787No Hit
GAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAG2329461.689529927640894No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1946721.4119331092772924No Hit
AGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGG1366930.9914182445674824No Hit
GGTGGTGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGG1301590.9440279114121347No Hit
AGGGGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGG910360.6602733959489171No Hit
AGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGG617930.44817735792292535No Hit
GAGGGGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGG608920.441642510942061No Hit
CGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGG585740.42483032969717327No Hit
TGGGGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGG555470.40287585487910826No Hit
TGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGGCG552020.4003736104746707No Hit
AGGGGGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGG519970.3771281226015625No Hit
TAGGGGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGG500980.3633548990536584No Hit
TGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGG494680.3587855831846855No Hit
CGTTGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG461290.3345682090791291No Hit
GCCAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCGGCC453780.32912129444802013No Hit
GAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGG442950.32126642288278573No Hit
AAGGGGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGG425160.308363545214686No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT416930.3023944230556944No Hit
GAGGGGGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCG371090.2691472104471678No Hit
GGAGGGGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCG361850.26244554717267415No Hit
GGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGTAA360480.26145190229323084No Hit
ATGGGGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGG343160.24888991009472122No Hit
TGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGA334010.24225352276121292No Hit
CAGGGGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGG328350.238148391361469No Hit
TAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAG321670.23330346596389137No Hit
GTGGTGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGGC320400.23238234990776507No Hit
TTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGGC318490.2309970493824098No Hit
TGGGGGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGG286470.20777332015629674No Hit
GTTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGG267710.19416691290202184No Hit
TGAGGGGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCG265030.1922231404371254No Hit
TTGGGGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGG259110.18792943409675722No Hit
AGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGA254030.18424496987225208No Hit
TAGGGGGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCG243480.1765931790123054No Hit
TGGGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGGCGGGCCCGGGCGG242810.1761072358960813No Hit
GTGGGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGG242450.17584613213213998No Hit
GATGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGG232790.16883984779971484No Hit
AAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAG230220.1669758570404672No Hit
TGTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG213670.15497233678149866No Hit
ATGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGGC211950.15372484102044573No Hit
CAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAG208020.15087445826408644No Hit
CTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGGC203280.1474365920388592No Hit
GGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCGGCCCCCTTTCCGGCCGCACCCCGTTTCCCA201330.1460222799841771No Hit
AGGGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGGCGGGCCCGGGCGG200960.1457539233379041No Hit
AAGGGGGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCG200050.14509391104571912No Hit
GCGCCAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCGG196640.1426206781706084No Hit
CGCGGGGAAGAGGGGAGGGCGGGCCCGGGCGGAAAGGACGGGGGGTCTCC195710.14194616011376002No Hit
TTGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAG195080.14148922852686271No Hit
GTGGGGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGG192990.13997337611953678No Hit
GTGGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGGCGGGCCCGGGCGG192360.13951644453263948No Hit
TGTGGTGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGG188330.13659353295296317No Hit
ATGGGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGG187390.13591176201378308No Hit
GGGCCCCGCGGGGAAGAGGGGAGGGCGGGCCCGGGCGGAAAGGACGGGGG182400.13229257373026326No Hit
GGGGCCCCGCGGGGAAGAGGGGAGGGCGGGCCCGGGCGGAAAGGACGGGG178370.12936966215058696No Hit
GGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGG175920.1275927059793197No Hit
ATGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAG174150.12630894580660826No Hit
CGAGGGGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCG171860.12464803575264827No Hit
TGTTGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG163570.11863539629966646No Hit
GTGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAG163360.1184830857707007No Hit
CGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGA163270.11841780982971538No Hit
CGTCCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGGC152460.1105774440291444No Hit
CAGGGGGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCG147250.10679869233432711No Hit
TGAGGGGGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGC146610.10633450786509814No Hit
CTGGGGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGG145760.10571801286690338No Hit
TGCCAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCGGC141300.10248322734696383No Hit
GGGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAG140080.10159837570249605No Hit
GGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTACCT138120.10017681076548227No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)