FastQCFastQC Report
Wed 28 Jun 2023
EGAF00008086178

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008086178
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14449739
Sequences flagged as poor quality0
Sequence length35-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN4816313.3331467094319147No Hit
GGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGG2312291.600229595842527No Hit
GTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGGC763900.5286600678392877No Hit
GGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGGCGG724470.5013723777294524No Hit
TGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGG519040.35920371987341776No Hit
GAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAG459770.31818567795584407No Hit
GCCAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCGGCC286000.19792745045429538No Hit
AGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGG270550.18723521580562805No Hit
GGTGGTGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGG230760.15969838624766855No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT217740.15068784287384016No Hit
AGGGGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGG163460.11312315052887806No Hit
GGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCGGCCCCCTTTCCGGCCGCACCCCGTTTCCCA149810.10367661312083215No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)