FastQCFastQC Report
Wed 28 Jun 2023
EGAF00008086183

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008086183
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12404118
Sequences flagged as poor quality0
Sequence length35-76
%GC73

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2309381.8617849330359482No Hit
GGGGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTC1037550.8364560865996277No Hit
GGTGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTC764210.6160937843383947No Hit
GTGGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTC633270.5105320668507023No Hit
GTTGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTC605660.48827332987319216No Hit
TTTGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTC497110.40076206949982257No Hit
TGTGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTC495590.3995366699994308No Hit
TGGGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTC430310.3469089861931336No Hit
GGGAGCCGGGTCCCCGTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCC393770.317451027150822No Hit
TTGGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTC384910.3103082379577492No Hit
GTGAGCCGGGTCCCCGTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCC328480.2648152815057064No Hit
GGGGGGTCGGTCGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGG319150.25729358588817036No Hit
TTTGGGTCGGTCGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGG309070.2491672523592568No Hit
GGTGGGTCGGTCGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGG306450.24705505058884475No Hit
GTTGGGTCGGTCGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGG306170.24682931910193048No Hit
GAGGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTC290380.23409967560772965No Hit
GTGGGGTCGGTCGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGG262900.21194574253485818No Hit
TGTGGGTCGGTCGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGG255640.206092847552724No Hit
TTGAGCCGGGTCCCCGTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCC253320.20422250094686298No Hit
GCGGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTC240300.19372598680534964No Hit
GATGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTC231080.18629297141481563No Hit
TGGAGCCGGGTCCCCGTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCC228940.18456773790768516No Hit
CGGGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTC213120.1718139088970292No Hit
TTGGGGTCGGTCGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGG203620.1641551620195809No Hit
AGTGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTC196280.15823777232689984No Hit
AGGGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTC193130.15569829309911434No Hit
TATGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTC188840.15223976424603508No Hit
AGGAGCCGGGTCCCCGTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCC187800.15140133300892494No Hit
TGGGGGTCGGTCGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGG184710.14891022481404967No Hit
GGGGTGGTGGTGGCGCGCGGGCGGGGCCGGGGGTGGGGTCGGCGGGGGAC178190.14365390590447463No Hit
ATTGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTC174620.14077582944631775No Hit
TTTAGCCGGGTCCCCGTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCC174450.14063877818640552No Hit
GCTGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTC174200.14043723221594634No Hit
GGGGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTCGGAACGGAAC169960.13701901255695892No Hit
TAGGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTC169420.13658367326076712No Hit
GGGGGTGGCGCGCGGGCGGGGCCGGGGGTGGGGTCGGCGGGGGACCGCCC168400.1357613657012937No Hit
GGTGTGGTGGTGGCGCGCGGGCGGGGCCGGGGGTGGGGTCGGCGGGGGAC168280.1356646236354733No Hit
GTTAGCCGGGTCCCCGTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCC168170.1355759434084713No Hit
GGTAGCCGGGTCCCCGTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCC168040.13547113950383252No Hit
CGGAGCCGGGTCCCCGTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCC163870.13210935271657365No Hit
CGTGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTC160920.1297311102651555No Hit
ATGAGCCGGGTCCCCGTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCC159310.12843315421539847No Hit
GGTGGTGGCGCGCGGGCGGGGCCGGGGGTGGGGTCGGCGGGGGACCGCCC157750.12717550735973326No Hit
GTTGTGGTGGTGGCGCGCGGGCGGGGCCGGGGGTGGGGTCGGCGGGGGAC154500.12455540974376414No Hit
ATGGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTC150960.12170151880206234No Hit
TTTGTGGTGGTGGCGCGCGGGCGGGGCCGGGGGTGGGGTCGGCGGGGGAC150100.12100820066368281No Hit
GTTGGTGGCGCGCGGGCGGGGCCGGGGGTGGGGTCGGCGGGGGACCGCCC147530.11893630808736261No Hit
CTGGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTC143500.11568738704356085No Hit
TGTAGCCGGGTCCCCGTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCC141520.11409114295752426No Hit
GTGGTGGTGGTGGCGCGCGGGCGGGGCCGGGGGTGGGGTCGGCGGGGGAC140610.11335751562505289No Hit
TTTGGTGGCGCGCGGGCGGGGCCGGGGGTGGGGTCGGCGGGGGACCGCCC140390.11318015517104885No Hit
GGGGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTCGGAA136070.10969744080151446No Hit
TGTGTGGTGGTGGCGCGCGGGCGGGGCCGGGGGTGGGGTCGGCGGGGGAC135890.10955232770278388No Hit
GTGGGTGGCGCGCGGGCGGGGCCGGGGGTGGGGTCGGCGGGGGACCGCCC131660.1061421698826148No Hit
CTTGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTC130580.10527149129023118No Hit
AGTGGGTCGGTCGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGG128710.10376392743119663No Hit
AGGGGGTCGGTCGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGG125750.10137762314096012No Hit
AGTGTGGTGGTGGCGCGCGGGCGGGGCCGGGGGTGGGGTCGGCGGGGGAC125610.10126475739750299No Hit
GGTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTCGGAACGGAAC125430.1011196442987724No Hit
TGTGGTGGCGCGCGGGCGGGGCCGGGGGTGGGGTCGGCGGGGGACCGCCC125130.1008777891342214No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)