FastQCFastQC Report
Wed 28 Jun 2023
EGAF00008086186

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008086186
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12883416
Sequences flagged as poor quality0
Sequence length35-76
%GC72

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGTAA4969503.857284434500912No Hit
GGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGG2288411.7762447475110639No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2106201.6348148658709771No Hit
CGTTGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG1589531.2337799229645305No Hit
GGTTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGTAA1257920.9763870079177759No Hit
GTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGGC886880.6883888558748705No Hit
GGCTTTCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGTAA816840.6340243922885048No Hit
CTGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGT785730.6098770698702891No Hit
TGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGTA770800.5982885284461823No Hit
TGTTGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG770700.5982109092805822No Hit
TGTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG759500.589517562733362No Hit
GGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGGCGG707410.5490857393722286No Hit
GAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAG699610.5430314444554146No Hit
TCTGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTG569940.4423826724216621No Hit
TGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGG499530.3877310179225758No Hit
CGTCTCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG475830.3693352756753333No Hit
CGTCACGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG456470.3543082052151386No Hit
CGTCCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGGC430360.3340418410769318No Hit
ACTCGTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACC394250.30601356037870703No Hit
GCCAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCGGCC389600.30240426917829866No Hit
CGTTGTGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG387140.3004948377045343No Hit
CTTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG349710.27144198402038716No Hit
ACGTGCGTTCAACGTGACGGGCGAGAGGGCGGCCCCCTTTCCGGCCGCAC311990.24216403475599949No Hit
GCGTTCAACGTGACGGGCGAGAGGGCGGCCCCCTTTCCGGCCGCACCCCGTTTCCCAAGACGAACGGCTCTCCGC311970.24214851092287945No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT311130.24149650993183797No Hit
CGCGGGGAAGAGGGGAGGGCGGGCCCGGGCGGAAAGGACGGGGGGTCTCC291450.22622105814172266No Hit
CGTTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCG280250.2175277115945026No Hit
AGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGG269260.20899736529504287No Hit
GGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGTA258650.20076197182486386No Hit
CCCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGAGAG251100.19490172482205032No Hit
GTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGGC247640.1922161016922841No Hit
GGCTCCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGTAA240290.1865110930206709No Hit
CTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGGC239400.1858202824468293No Hit
AGGGGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGG233570.18129508509233885No Hit
GGCTACCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGTAA230760.17911398653897384No Hit
GGCCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGTA228450.17732098381360967No Hit
ATCTGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCT218290.16943487658863146No Hit
CGTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG206700.16043881529557066No Hit
CGCCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG196270.15234313632347196No Hit
CGACGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG190410.14779465321930146No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCAT188560.14635869865569814No Hit
GGTGGTGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGG187580.1455980308328164No Hit
CTCGTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCC182880.14194993004960796No Hit
TCCCCCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGA182880.14194993004960796No Hit
CGTAGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG180950.1404518801535245No Hit
GGTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG177300.13761878060911795No Hit
GGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCGGCCCCCTTTCCGGCCGCACCCCGTTTCCCA175710.1363846358760751No Hit
CGTGCGTTCAACGTGACGGGCGAGAGGGCGGCCCCCTTTCCGGCCGCACC170100.13203020068590504No Hit
GAGGGGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGG169760.13176629552286442No Hit
TCGTTGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCG168640.13089696086814243No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTTAGTGCGGACACCCG167820.13026048371022095No Hit
AGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGG166780.1294532443879791No Hit
CGTGGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG162130.12584395318757075No Hit
TGTCCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGGC160390.12449337970612763No Hit
TGCCAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCGGC157040.12189313765852162No Hit
CCCCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGAGA155550.12073661209107894No Hit
CCCCACACGCGCGGGACACGCCCGCCCGCCCCCGCCACGCACCTCGGGAG154300.11976637252107672No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG152990.11874956145171435No Hit
TCCCCACCGGGCCTTCCCGGCCGTCCCGGAGCCGGTCGCGGCGCACCGCC152710.11853222778803385No Hit
GGCTGCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGTAA151860.11787246488043233No Hit
GTGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGTA151240.11739122605371122No Hit
GCCCGCCCGCCCCCGCCACGCACCTCGGGAGAGGCGATGGGGGGGTGGAG148420.11520236558378617No Hit
GCGGGGAAGAGGGGAGGGCGGGCCCGGGCGGAAAGGACGGGGGGTCTCCC147530.11451155500994457No Hit
CGCTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCG146490.11370431568770271No Hit
GTTCAACGTGACGGGCGAGAGGGCGGCCCCCTTTCCGGCCGCACCCCGTT143070.11104974022417657No Hit
TGTGCGTTCAACGTGACGGGCGAGAGGGCGGCCCCCTTTCCGGCCGCACC140410.10898507041921181No Hit
GTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGGCGCGCGGAGGGGAG138670.10763449693776868No Hit
AGGGGGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGG135900.10548444605064371No Hit
GGCATCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGTAA134820.10464615906216179No Hit
GGCCTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGTAA134420.10433568239976107No Hit
TAGGGGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGG133840.10388549123928001No Hit
AACGTGACGGGCGAGAGGGCGGCCCCCTTTCCGGCCGCACCCCGTTTCCC133420.10355949074375927No Hit
TCAACGTGACGGGCGAGAGGGCGGCCCCCTTTCCGGCCGCACCCCGTTTC130600.10137063027383421No Hit
TGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGGCG130560.10133958260759414No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)