FastQCFastQC Report
Wed 28 Jun 2023
EGAF00008086199

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008086199
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12711948
Sequences flagged as poor quality0
Sequence length35-76
%GC63

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGTCGGTCGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGG2263391.7805217579555863No Hit
GTTGGGTCGGTCGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGG2205081.7346515262648965No Hit
GGTGGGTCGGTCGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGG2157011.6968367082684732No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2156811.696679375969757No Hit
TTTGGGTCGGTCGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGG2142381.6853278506173877No Hit
GTGGGGTCGGTCGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGG1936371.5232677163248307No Hit
TGTGGGTCGGTCGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGG1713671.3480782017044124No Hit
TTGGGGTCGGTCGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGG1444481.1363167942474277No Hit
TGGGGGTCGGTCGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGG1265520.9955358533562284No Hit
AGGGGGTCGGTCGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGG1240460.9758221163270964No Hit
CGGGGGTCGGTCGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGG1102380.8671998972934754No Hit
AGTGGGTCGGTCGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGG1052020.827583624476752No Hit
GAGGGGTCGGTCGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGG900980.7087662724863254No Hit
ATTGGGTCGGTCGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGG895520.7044711007313749No Hit
ATGGGGTCGGTCGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGG883050.6946614319064238No Hit
AAGGGGTCGGTCGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGG765070.6018511088937746No Hit
TATGGGTCGGTCGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGG642800.5056660080736642No Hit
TAGGGGTCGGTCGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGG615540.4842216157586548No Hit
GATGGGTCGGTCGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGG610130.4799657770783832No Hit
CGTGGGTCGGTCGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGG594670.4678039903876259No Hit
CTGGGGTCGGTCGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGG568550.44725639217529833No Hit
AATGGGTCGGTCGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGG550550.43309648529084604No Hit
GCGGGGTCGGTCGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGG501040.3941488747436664No Hit
CAGGGGTCGGTCGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGG488590.3843549391485868No Hit
ACGGGGTCGGTCGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGG419650.33012249578113445No Hit
CTTGGGTCGGTCGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGG403430.3173628463552557No Hit
CCGGGGTCGGTCGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGG383470.30166108294338523No Hit
TCGGGGTCGGTCGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGG269190.21176140745698457No Hit
ACTGGGTCGGTCGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGG266390.20955875527495865No Hit
GCTGGGTCGGTCGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGG262340.20637277622595687No Hit
GGGGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGGCTGGACGAG254130.19991428536365943No Hit
CATGGGTCGGTCGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGG249170.19601244435549925No Hit
TCTGGGTCGGTCGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGG238040.18725690193194622No Hit
GTTGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGGCTGGACGAG220940.17380499039171654No Hit
GGTGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGGCTGGACGAG219410.17260139830653806No Hit
GTGGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGGCTGGACGAG204650.16099027466128715No Hit
TTTGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGGCTGGACGAG201540.15854375741625124No Hit
TTTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA193890.15252579699035898No Hit
TGTGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGGCTGGACGAG168690.13270192735212574No Hit
CCTGGGTCGGTCGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGG163560.12866635389005682No Hit
AGGGGTCGGTCGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGGC149870.11789695804293725No Hit
TTGGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGGCTGGACGAG144050.11331858815029766No Hit
AGGGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGGCTGGACGAG137830.1084255536602258No Hit
CGGGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGGCTGGACGAG134970.10617570178858503No Hit
TGGGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGGCTGGACGAG134780.10602623610480472No Hit
CGGGGTCGGTCGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGGC131480.10343025317598846No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)