FastQCFastQC Report
Wed 28 Jun 2023
EGAF00008086204

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008086204
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25003990
Sequences flagged as poor quality0
Sequence length35-101
%GC67

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGG363135514.52310211290278No Hit
GTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGGC6015812.405940011974089No Hit
GGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGGCGG5187342.0746048930590675No Hit
TGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGG4661901.8644624317958853No Hit
GAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAG3279241.3114866867247987No Hit
GGTGGTGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGG3175281.2699093224721336No Hit
AGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGG2133610.8533078120731931No Hit
GGGGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGG1967220.7867624327157386No Hit
AGGGGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGG1805980.7222767246347483No Hit
GGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGTAA1738170.6951570529343517No Hit
GAGGGGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGG1290770.5162256103925814No Hit
TGGGGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGG1286550.5145378797543911No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1196510.47852762699073226No Hit
TGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGGCG1130480.45211984167326896No Hit
GCCAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCGGCC973120.38918588593260517No Hit
CGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGG929480.3717326714656341No Hit
TAGGGGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGG880330.35207580870093136No Hit
AGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGG838600.3353864723190179No Hit
CGTTGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG753460.30133590678927646No Hit
GCGCCAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCGG746180.2984243714703133No Hit
AAGGGGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGG717300.2868742148753059No Hit
GGAGGGGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCG703430.28132710019480894No Hit
TGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGG637080.25479133530288567No Hit
GAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGG617560.24698458126083078No Hit
CAGGGGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGG599730.23985371934639232No Hit
TGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGA544130.21761726828398187No Hit
GGGGGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGG528870.2115142423269246No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT523540.20938258253982664No Hit
TGAGGGGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCG522150.2088266712632664No Hit
ATGGGGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGG520910.2083307504122342No Hit
GGCTGCTAGGCGCCGGCCGAGGCGAGGCGCCGCGCGGAAAACCGCGGCCC497880.19912022041282212No Hit
TGGGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGG488910.19553279296624257No Hit
GTTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGG447600.17901142977580778No Hit
TTGGGGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGG428930.17154462147841204No Hit
GGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCGGCCCCCT425600.17021283403168855No Hit
TTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGGC424300.16969291701044514No Hit
TAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAG418440.1673492910531479No Hit
AGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGA400700.16025442339402632No Hit
GTGGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGG399530.15978649807490725No Hit
GATGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGG387300.15489527871351733No Hit
AGGGGGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGG381750.15267563296897815No Hit
GTGGGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGG378790.15149182190522392No Hit
GTGGTGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGGC369030.14758844488419648No Hit
GGGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGA361020.1443849561609967No Hit
GTGGGGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGG358380.1433291246717024No Hit
CGAGGGGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCG347290.13889383254432594No Hit
AGGGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGG338270.13528640828923702No Hit
TGTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG330290.1320949176511429No Hit
GGTGGGGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCG308090.12321633467298619No Hit
ACTCGTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACC305120.12202852424753009No Hit
TGCCAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCGGC294860.11792517914140904No Hit
AAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAG276360.11052635999294512No Hit
CAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAG273200.10926256169515346No Hit
ATGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGGC270960.10836670467393404No Hit
CTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGGC269010.10758682914206892No Hit
CGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGA266790.10669897084425327No Hit
GGTTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGTAA264410.10574712275920764No Hit
GGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGG263520.10539117956774098No Hit
TGGGGGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGG261570.10461130403587586No Hit
ATGGGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGG258510.10338749935510291No Hit
GAGGGGGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCG258440.10335950382318981No Hit
CCTTGGTCCGTGTTACAAGACGGGTCGGGTGGGTAGCCGACATCGCCGCC253870.10153179552543415No Hit
GGGGCCCCGCGGGGAAGAGGGGAGGGCGGGCCCGGGCGGAAAGGACGGGG253690.10145980701480044No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)