FastQCFastQC Report
Wed 28 Jun 2023
EGAF00008086206

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008086206
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29342761
Sequences flagged as poor quality0
Sequence length35-101
%GC74

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGG435813114.852491215806173No Hit
GTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGGC8197112.793571470660174No Hit
GGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGGCGG7046122.401314586585768No Hit
TGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGG5855431.995527960030755No Hit
GCCAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCGGCC4822511.6435092798527038No Hit
GAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAG4142921.411905307752055No Hit
GCGCCAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCGG3268771.1139953735096706No Hit
GGTGGTGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGG3262611.111896048228045No Hit
CGTTGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG3233361.1019276611359101No Hit
AGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGG2680300.9134450571982644No Hit
GGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCGGCCCCCT2521580.8593533512405325No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2483070.8462291602347851No Hit
TGCCAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCGGC1849030.6301486080331704No Hit
AGGGGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGG1735260.5913758422392494No Hit
TGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGGCG1615630.5506059910313144No Hit
ACTCGTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACC1504400.5126988561165052No Hit
TGTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG1444510.49228837054563473No Hit
GAGGGGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGG1399660.47700351033769456No Hit
GGGGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGG1318370.4492999142105271No Hit
CGCCAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCGGC1274630.43439334151274994No Hit
GGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGTAA1261420.4298913793422507No Hit
CCAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCGGCCC1209910.4123367940733321No Hit
CGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGG1200260.4090480783318243No Hit
AGCCAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCGGC1108780.3778717347014482No Hit
AGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGG1102690.37579626538893185No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT876950.29886417300675966No Hit
TAGGGGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGG868510.2959878247312855No Hit
TGTTGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG830380.282993137557846No Hit
TGGGGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGG801940.2733007981082625No Hit
GGAGGGGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCG791290.2696712828080493No Hit
TGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGG781330.2662769192033429No Hit
CGTCCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGGC752950.2566050277272817No Hit
AAGGGGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGG725800.24735231970842825No Hit
GCGTTCAACGTGACGGGCGAGAGGGCGGCCCCCTTTCCGGCCGCACCCCG711960.2426356538159446No Hit
AGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCGGCCCCC682760.23268430670174492No Hit
GTGCCAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCGG681020.23209131546959744No Hit
ACGTGCGTTCAACGTGACGGGCGAGAGGGCGGCCCCCTTTCCGGCCGCAC619490.21112191862245003No Hit
CGTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG605480.2063473168049864No Hit
CAGGGGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGG593720.20233951399461012No Hit
CAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCGGCCCC593090.20212481027262566No Hit
GAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGG587580.20024700470415857No Hit
TGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGA584560.19921779003686807No Hit
CGTTGTGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG584290.19912577415601754No Hit
TTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGGC580450.19781710385058854No Hit
TGAGGGGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCG580410.19780347186824035No Hit
GTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGGC565960.19287891824494635No Hit
CGTCTCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG558870.19046264937372456No Hit
GTTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGG532660.1815302929400543No Hit
GGCCAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCGGC526940.179580919464259No Hit
CGTCACGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG524990.17891636032478336No Hit
GGTGGCGACGACGCGGGGGACAACGGGGCCCCGCGGGGAAGAGGGGAGGG510060.1738282229133107No Hit
ATGGGGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGG499760.1703179874586444No Hit
TAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAG491080.16735984728908096No Hit
GCCGGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCGGCC477960.1628885570788652No Hit
GCGCTAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCGG476110.1622580778952601No Hit
GGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTACCT475220.16195476628801223No Hit
GATGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGG468170.15955212939913868No Hit
AGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGA459720.15667237312807747No Hit
TGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCGGCCCCC446400.15213292300612066No Hit
GTGGTGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGGC445430.15180234743417637No Hit
TCGCCAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCGG435180.14830915195744532No Hit
CGCCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG433370.14769230475618841No Hit
TGCGCCAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCG421030.14348683820176295No Hit
CGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGGCGC416970.14210319199341875No Hit
CTTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG409680.13961876321045588No Hit
GGTGGGGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCG399160.13603355185287438No Hit
TTGGGGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGG389780.1328368519922171No Hit
CGAGGGGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCG389060.13259147630994914No Hit
TACTCGTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAAC387130.1319337331616476No Hit
GTGGGGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGG378020.1288290491818408No Hit
ACGCCAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCGG374580.127656698699894No Hit
ATGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGGC373000.12711823539713935No Hit
GGGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAG371330.12654910013410123No Hit
CTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGGC368860.1257073252240987No Hit
GGGGGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGG354910.12095317138015745No Hit
GTGGGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGG344170.11729298411966073No Hit
GTGGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGG342340.1166693209272297No Hit
CTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGGC340890.11617516156710679No Hit
CAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAG336650.1147301714381956No Hit
CGTGCGTTCAACGTGACGGGCGAGAGGGCGGCCCCCTTTCCGGCCGCACC335550.1143552919236196No Hit
AAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAG334380.11395655643993419No Hit
AGGGGGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGG332120.11318634943725984No Hit
CCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCGGCCCCCTTTCC330840.11275012600211684No Hit
GGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGG322410.10987718572222976No Hit
GTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCGGCCCCCTT319910.10902518682546608No Hit
CTCGTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCC316020.10769947654210182No Hit
TTGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAG314740.10726325310695883No Hit
GGTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG312820.10660891795424433No Hit
GGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGGCGGGCCCGGGCGGAA307760.10488447218719466No Hit
GTGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAG305600.10414834514039084No Hit
GGGGCCCCGCGGGGAAGAGGGGAGGGCGGGCCCGGGCGGAAAGGACGGGG301940.10290101875552883No Hit
TGGGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGG297150.10126858886932964No Hit
GGGCCCCGCGGGGAAGAGGGGAGGGCGGGCCCGGGCGGAAAGGACGGGGG296000.10087666937681837No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)